摘要
目的 观察绿原酸通过调控TLR4/NF-κB信号通路减轻脓毒症(sepsis)大鼠肠道氧化应激损伤、改善肠上皮细胞屏障功能的作用。方法 选取SD大鼠60只,采用随机数字表法分成假手术组、模型对照组、绿原酸(低、高剂量)组,每组15只。术后24 h,应用酶联免疫吸附法(ELISA)检测大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、IL-10;比色法测定小肠组织中一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平;采用Western blotting法测定小肠组织中的蛋白表达[密封蛋白-1(Claudin-1)、闭锁连接蛋白-1(ZO-1)及TLR-4/NF-κB通路相关的蛋白]。结果 与模型对照组[(4.45±0.14)分]比较,低剂量组[(4.17±0.22)分]、高剂量组[(1.95±0.08)分]Chiu评分明显下降(t=4.159、60.048,均P<0.001);高剂量组血清TNF-α、IL-6、IL-10水平及小肠组织中NO、MDA含量均显著降低(均P<0.05),SOD含量、Claudin-1、ZO-1蛋白表达量及MyD88、TLR-4蛋白与p-NF-κB p65(Ser536)水平均显著升高(均P<0.05)。结论 绿原酸可经抑制TLR4/NF-kB信号转导途径,减轻脓毒症大鼠的炎症反应程度及小肠组织的氧化应激损伤,进而促进大鼠的肠道屏障功能改善。
Objective To observe the effect of chlorogenic acid on reducing intestinal oxidative stress injury and improving intestinal cell barrier function through TLR4/NF-κB signaling pathway in septic rats.Methods Sixty SD rats were randomly divided into four groups by the random number table method: sham operation group,model control group and chlorogenic acid(low and high dose) group,with 15 rats in each group.24 hours after operation,the levels of tumor necrosis factor-α(TNF-α),interleukin(IL-6) and IL-10 were detected by enzyme-linked immunosorbent assay(ELISA),the levels of nitric oxide(NO),superoxide dismutase(SOD) and malondialdehyde(MDA) in small intestine were measured by colorimetry,and western blot was used to detect claudin-1,ZO-1 and TLR-4/NF-κB pathway related protein expression.Results Compared with the model control group,Chiu score in low dose group(4.17±0.22) and high dose group(1.95±0.08) decreased significantly(t=4.159,60.048,all P<0.01).The levels of TNF-α,IL-6,IL-10 and the contents of NO and MDA in the small intestine in high dose group were significantly decreased(all P<0.05),the content of SOD,the expression of Claudin-1,ZO-1 protein,MyD88,TLR-4 protein and p-NF-κB p65(Ser536) increased significantly(all P<0.05).Conclusion Chlorogenic acid can reduce serum inflammatory response,reduce oxidative damage of small intestine in septic rats through inhibiting TLR4/NF-κB signal transduction,and then improve intestinal barrier function.
作者
王金鑫
饶春晖
代波
俞慧琴
汪志刚
WANG Jinxin;RAO Chunhui;DAI Bo;YU Huiqin;WANG Zhigang(Department of Gastroenterology,Zhejiang Medical Health Group Hangzhou Hospital,Hangzhou,Zhejiang 310022,China;不详)
出处
《中华全科医学》
2023年第3期405-408,共4页
Chinese Journal of General Practice
基金
浙江省医药卫生科技计划项目(2019KY522)。
