摘要
采用15K SNP芯片数据分析了15份黑、紫色小麦种质资源的遗传差异,利用HPLC法对15份小麦种质资源籽粒中的6种花青素含量进行测定,为黑、紫色小麦杂交育种亲本选择提供理论依据。结果表明,通过SNP芯片数据分析共获得7 116个有效SNP(单核苷酸多态性)位点,SNP多态性比率为77.12%,每条染色体上有128~682个SNP位点,在A、B、D染色体组中不均匀分布。各染色体组中SNP标记的数量表现为B>A>D,其中,3B染色体上SNP位点最多,4D染色体上SNP位点最少。黑、紫色小麦种质资源籽粒中总花青素含量均显著高于普通小麦,不同种质资源籽粒中花青素含量差异很大。紫色小麦、卫辉黑小麦和周黑麦1号籽粒中花青素种类丰富而且总含量高于其他种质资源。蜀紫麦1801、特色小麦1、特色小麦2、漯珍1号、豫州黑麦1号和豫州黑麦2号籽粒中总花青素含量较低。15份黑、紫色小麦种质资源可分为Ⅰ、Ⅱ、Ⅲ3类,3类种质资源籽粒中花青素含量差异很大,表现为Ⅱ>Ⅲ>Ⅰ,而且遗传差异大的种质资源间花青素含量差异大。综上,紫色小麦、卫辉黑小麦和周黑麦1号总花青素含量高,可与遗传相似度低的其他材料进行杂交育种亲本组配。
In order to provide a theoretical basis for the selection of parents in black or purple wheat crossbreeding,the genetic differences of 15 black or purple wheat germplasm resources were analyzed on the basis of 15K SNP chip data,and HPLC method was used to test the contents of six anthocyanins in seeds of 15 germplasm resources. The results showed that a total of 7 116 effective single nucleotide polymorphism(SNP)loci were obtained from SNP chip data,and 77. 12% of the SNP loci were polymorphic among germplasm resources. There were 128—682 SNP loci on each chromosome,which were unevenly distributed in A,B and D genomes. The genome distribution order of SNP loci number was B>A>D,chromosome 3B had the most SNP loci number,and chromosome 4D had the least SNP loci number. The total anthocyanin content in the seeds of black and purple wheat germplasm resources was significantly higher than that of common wheats,and there were big differences in seed anthocyanin contents among 15wheat germplasm resources. The seed of Zisexiaomai,Weihuiheixiaomai and Zhouheimai 1 contained more kinds of anthocyanin and higher total anthocyanin content than the other germplasm resources. The seed of Shuzimai 1801,Tesexiaomai 1,Tesexiaomai 2,Luozhen 1,Yuzhouheimai 1 and Yuzhouheimai 2 contained lower total anthocyanin content. The 15 wheat germplasm resources could be classified into three groups,marked as Ⅰ,Ⅱ and Ⅲ. The anthocyanin contents of the three classes were very different,showing Ⅱ>Ⅲ>Ⅰ,and the anthocyanin contents of the germplasm resources with great genetic differences were very different. In conclusion,Zisexiaomai,Weihuiheixiaomai and Zhouheimai 1 had high anthocyanin contents,and could hybridize with other germplasm resources with low genetic similarities for breeding.
作者
杨丽娟
付亮
任星旭
朱坤
蒋志凯
YANG Lijuan;FU Liang;REN Xingxu;ZHU Kun;JIANG Zhikai(Xinxiang Academy of Agricultural Sciences,Henan Province/Henan Province Strong Gluten Wheat Breeding Engineering Technology Research Center,Xinxiang 453000,China)
出处
《河南农业科学》
北大核心
2022年第12期28-36,共9页
Journal of Henan Agricultural Sciences
基金
河南省科技攻关项目(212102110301)
国家现代农业产业技术体系项目(CARS-3-2-34)。
