摘要
FAH超家族是由胡萝卜素羟化酶、脂肪酸和甾醇去饱和酶组成的一类蛋白质。类胡萝卜素含量是小麦营养价值的主要指标之一。本研究利用生物信息学的方法在小麦全基因组水平一共鉴定到96个FAH基因,并预测了TaFAH蛋白的理化性质。结果表明,96个FAH基因在小麦染色体上分布不均匀,在A、B、D染色体组以及未锚定到染色体的Contigs上分别有35、26、34和1个FAH基因。遗传进化分析可以将小麦TaFAH基因划分为五个亚家族,相同亚族的基因结构和保守结构域基本一致。顺式作用元件分析发现小麦TaFAH基因上游2000 bp的启动子序列中共发现18种类型的顺式作用元件,大部分基因与光响应元件、脱落酸响应元件以及茉莉酸甲酯响应元件有关。96个基因中鉴定出14个小麦TaFAH基因为串联重复基因,80个小麦TaFAH基因为片段重复基因。表达模式分析发现,TaFAH基因在不同组织、不同生长发育时期和不同非生物胁迫环境下存在差异表达,序列相似性高的基因,尤其是同源基因具有相似的表达模式。本研究对小麦TaFAH基因超家族进行鉴定和分析,为进一步研究小麦TaFAH基因超家族的功能提供了一定的参考和依据。
FAH superfamily is composed of carotene hydroxylase,fatty acid and sterol desaturase.Carotenoid content is one of the main indexes of wheat nutritional value.In this study,96 FAH genes were identified by bioinformatics methods at the whole genome level of wheat,and the physicochemical properties of TaFAH proteins were predicted.The results showed that 96 FAH genes were inevenly distributed in wheat chromosomes,and 35,26,34 and 1 FAH genes were in A,B and D genomes and in contigs that were not anchored to chromosomes.Genetic evolution analysis can divide wheat TaF AH genes into five subfamilies,and the genes structure and conserved domain of the same subfamily are basically the same.cis-acting element analysis revealed that 18 types of cis-acting elements were found in the promoter sequence of 2000 bp upstream of TaFAH genes in wheat,most of which were related to photoresponse elements,exosalic acid response elements and methyl jasmonate response elements.Among the 96 genes,14 wheat TaFAH genes were identified as tandem repeats,and 80 wheat TaFAH genes were identified as fragment repeats.Analysis of expression patterns revealed that TaFAH genes was differentially expressed in different tissues,different growth and development periods and different abiotic stress environments,and genes with high similarity in sequence,especially homologous genes,had similar expression patterns.This study identified and analyzed the wheat TaFAH genes superfamily,providing certain reference and basis for further research on the function of the wheat TaFAH genes superfamily.
作者
丁睿
胡乐佳
郑晶
王其飞
朱靖环
华为
胡鑫
Ding Rui;Hu Lejia;Zheng Jing;Wang Qifei;Zhu Jinghuan;Hua Wei;Hu Xin(Zhejiang Key Laboratory of Agricultural Product Quality Improvement Technology,College of Agriculture and Food Science,Zhejiang A&F University,Hangzhou,311300;The Institute of Crops and Nuclear TechnologyUtilization of the Zhejiang Academy ofAgricultural Sciences,Hangzhou,310021)
出处
《分子植物育种》
CAS
北大核心
2022年第19期6276-6289,共14页
Molecular Plant Breeding
基金
国家重点研发计划项目(2018YFD0200500)
浙江省作物分子育种工程技术研究中心开放课题(2020KFKT)共同资助。
