摘要
【目的】明确不同产区白芷Angelica dahurica种源遗传差异特征,分析具有遗传差异的白芷种源在同圃栽培条件下的品质特征差异。【方法】应用相关序列扩增多态性(SRAP)分子标记分析不同白芷种源的遗传组成,以同圃栽培的不同种源白芷为材料,应用高效液相色谱法(HPLC)测定7种香豆素类化学成分,构建HPLC指纹图谱,进而分析不同种源的品质特征。【结果】非加权组平均法(UPGMA)聚类分析显示:在遗传相似系数为0.84处,部分河北产区的白芷种源与南方产区的白芷种源产生分离。同圃栽培下,仅应用7种香豆素类化学成分不能明确区分不同白芷种源,应用HPLC指纹图谱进行主成分分析(PCA)后,具有遗传差异的种源之间出现品质特征的区分。【结论】不同产区白芷种源遗传相似度较大,同圃栽培的部分浙江白芷种源的药材可以应用HPLC指纹图谱与其他产地种源进行区分。
[Objective]This study is to clarify the genetic differences of Angelica dahurica provenances in different producing areas in China,and analyze the quality characteristics of A.dahurica provenances with genetic differences under the same nursery cultivation conditions.[Method]The sequence-related amplified polymorphism(SRAP)molecular markers were used to analyze the genetic composition of different provenances of A.dahurica.Taking A.dahurica from different provenances cultivated in the same nursery as materials,the chemical components of 7 coumarins were determined by high performance liquid chromatography(HPLC),and HPLC fingerprints were constructed to analyze the quality characteristics of different provenances.[Result]Unweighted pair-group method with arithmetic means(UPGMA)showed that some provenances of A.dahurica from Hebei Province and those from South China were separated at the similarity coefficient of 0.84.Under the same nursery cultivation conditions,only 7 coumarins could not be used to clearly distinguish different provenances of A.dahurica.After principal component analysis(PCA)by HPLC fingerprint,the quality characteristics were differentiated between provenances.[Conclusion]The genetic similarity of A.dahurica provenances from different producing areas is relatively large,and the medicinal materials from some Zhejiang A.dahurica provenances cultivated in the same nursery can be distinguished from those from other producing areas by HPLC fingerprint.
作者
王丽赟
孙健
陈梦莹
姚辉
周水灯
王海阁
王盼
徐凯玠
王志安
WANG Liyun;SUN Jian;CHEN Mengying;YAO Hui;ZHOU Shuideng;WANG Haige;WANG Pan;XU Kaijie;WANG Zhian(Zhejiang Research Institute of Traditional Chinese Medicine Co.,LTD.,Hangzhou 310023,Zhejiang,China;College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China;Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Pan’an Traditional Chinese Medicine Industry Innovation and Development Institute,Pan’an 322399,Zhejiang,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2023年第1期30-37,共8页
Journal of Zhejiang A&F University
基金
磐安县中药材绿色发展示范项目(磐中药发202002)
国家现代农业产业技术体系资助
浙江省中药材新品种选育重大科技专项(2021C02074-4)
浙江省特色中药资源保护与创新利用重点实验室(2021E10013)
浙江省药用植物种质改良与质量控制技术重点实验室(2011E10015)。
关键词
白芷
SRAP
遗传
香豆素类
品质
Angelica dahurica
SRAP
genetic
coumarins
quality
