摘要
目的探讨年轻Sca-1骨髓干细胞对年老小鼠心外膜细胞衰老的调控作用及机制。方法将年轻和年老小鼠Sca-1骨髓干细胞分别与年老小鼠心外膜细胞共培养(分为Y Sca-1细胞组与O Sca-1细胞组)。通过细胞染色、基因及蛋白检测,探讨Sca-1骨髓干细胞对年老小鼠心外膜细胞衰老的影响。结果与O Sca-1细胞组相比,Y Sca-1细胞组可明显减少心外膜细胞衰老:P16免疫荧光染色阳性细胞比例下降;P27基因及蛋白表达减少;Sirt 1基因及蛋白表达增加(P<0.05)。分子机制研究发现,与O Sca-1细胞相比,Y Sca-1细胞可旁分泌更多的GDNF(P<0.05)。中和GDNF后,使Y Sca-1细胞失去减少年老心外膜细胞衰老的作用。结论年轻Sca1骨髓干细胞可通过旁分泌GDNF减少年老心外膜细胞衰老。
Objective To study the regulating effect of young Sca-1 bone marrow stem cell on epicardial cell senescence and its mechanism.Methods Young(Y)and old(O)Sca-1 bone marrow cell was co-cultured with old epicardial cell under hypoxia condition,respectively.The effect of Sca-1 bone marrow cell on epicardial cell senescence was detected by immunofluorescent staining,qRT-PCR,and western blot,respectively.Results Compared to O Sca-1 bone marrow cell,Y Sca-1 cell decreased epicardial cell senescence,with decreased senescent cell rate,decreased P27 gene and protein expression,and increased Sirt 1 gene and protein expression(P<0.05).More GDNF(Glial cell line-Derived Neurotrophic Factor)was found in Y Sca-1 cell group when compared to that in O Sca-1 cell group(P<0.05).The effect of Y Sca-1 cell on old epicardial cell senescence was lost when GDNF expression was blocked.Conclusion Young Sca-1 bone marrow stem cell decreases old epicardial cell senescence by GDNF.
作者
董珺
吕饶
高芳
于佳迪
李柳蓁
湛楚蓝
李月亮
刘慰华
黎佼
DONG Jun;Lü Rao;GAO Fang;YU Jiadi;LI Liuzhen;ZHAN Chulan;LI Yueliang;LIU Weihua;LI Jiao(The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2022年第23期2914-2918,共5页
The Journal of Practical Medicine
基金
广州市科技计划项目(编号:202002030336)
广州医科大学附属第六医院,清远市人民医院开放课题基金项目(编号:202201-304)
广东省基础与应用基础研究基金(编号:2019A1515011214,2021A1515011364)
广州医科大学附属第二医院临床研究项目(编号:2021-LCYJ-DZX-03,2022-LCYJ-YYDZX-03)
广州医科大学2022年度学生创新能力提升计划项目(编号:广医大发[2022]66号)。
关键词
干细胞
心外膜细胞
衰老
旁分泌
生长因子
stem cell
epicardial cell
senescence
paracrine
growth factor
