摘要
目的研究髓芯减压联合骨髓间充质干细胞对兔早期股骨头坏死的治疗作用。方法将48只新西兰白兔分为4组:空白组、模型组、髓芯减压组、髓芯减压联合骨髓间充质干细胞组。用液氮法构建兔股骨头坏死模型。运用病理学检测各组治疗情况,检测空骨陷窝率、骨小梁面积比、脂肪细胞平均直径。结果成功获得骨髓间充质干细胞,兔股骨头坏死模型构建成功。髓芯减压联合骨髓间充质干细胞治疗效果优于髓芯减压组,其治疗效果接近空白组。与模型组相比,治疗组(髓芯减压组和髓芯减压联合骨髓间充质干细胞组)的骨小梁面积比、脂肪细胞平均直径和空骨陷窝率差异有统计学意义(P<0.05),治疗有明显效果。其中髓芯减压联合骨髓间充质干细胞组的骨小梁面积比、脂肪细胞平均直径和空骨陷窝率分别是52.3%±3.5%、(35.1±3.6)μm和12.8%±2.5%。结论髓芯减压联合骨髓间充质干细胞治疗股骨头坏死具有显著疗效。
Objective To investigate the curative effect of core decompression combined with implantation of bone marrow mesenchymal stem cell(BMMSCs)on rabbit early stage femoral head necrosis.Methods Forty-eight healthy New Zealand rabbits were randomly and equally divided into 4 groups:blank group,model group,core decompression group and the core decompression combined with BMMSCs implantation.The early rabbit femoral head necrosis model was constructed by the liquid nitrogen method.The treatment situation in each group was examined by using the pathological method,including empty bone lacunae rate,bone trabecular area ratio,and the average diameter of fat cells.Results The bone marrow mesenchymal stem cell was achieved from rabbits and purity was higher.The model of early necrosis of rabbit′s femoral heads was successfully constructed.The best treatment method was core decompression combined with implantation of bone marrow mesenchymal stem cell compared with core decompression at early necrosis of femoral heads.Core decompression and core decompression combined with implantation of bone marrow mesenchymal stem cell Group compared with the model the area ratio of bone trabeculae was significantly increased while the average diameter of adipocyte cells and the rate of empty bone were significantly decreased.And the area ratio of bone trabeculae,the average diameter of adipocyte cells and the rate of empty bone were 52.3%±3.5%,(35.1±3.6)μm and 12.8%±2.5%,respectively.Conclusion The core decompression combined with BMMSCs implantation for treating femoral head necrosis has significant effect.
作者
李磊
郑怀亮
寇玉
张健
杨海韵
LI lei;ZHENG Huailiang;KOU Yu;ZHANG Jian;YANG Haiyun(Luoyang Bonesetting Hospital/Henan Provincial Orthopedic Hospital,Luoyang,Henan 471000,China;Medical College,Yangzhou University,Yangzhou,Jiangsu 225000,China;Affiliated Foshan Hospital of Traditional Chinese Medicine,Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong 528000,China)
出处
《重庆医学》
CAS
2018年第27期3490-3493,共4页
Chongqing medicine
基金
广东省自然基金资助项目(2014A030313771)
关键词
股骨头坏死
髓芯减压
骨髓间充质干细胞
病理学变化
femur head necrosis
core decompression
bone marrow mesenchymal stem cell
pathologic change
