摘要
本研究旨在比较屎肠球菌活菌和灭活菌对仔猪小肠紧密连接蛋白、细胞因子应答以及Toll样受体(TLR)基因表达的影响。试验选用健康的杜×长×大新生仔猪9窝,窝产仔数10-11头,随机分为对照组(CON组)、屎肠球菌活菌组(LB组)和屎肠球菌灭活菌组(HB组),每组3窝仔猪。从出生开始至6日龄,CON组、LB组和HB组仔猪分别经口灌服无菌脱脂乳、含屎肠球菌活菌(1×10^(8) CFU/mL)的脱脂乳和含屎肠球菌灭活菌(1×10^(8) CFU/mL)的脱脂乳,口服剂量为10 mL/(头•d)。仔猪于7日龄开始饲喂教槽料,21日龄断奶,并分别于这2个日龄从每窝中选出1头接近平均体重的仔猪屠宰取样,检测小肠黏膜紧密连接蛋白和细胞因子应答基因表达及分泌量,以及Toll样受体基因的表达。结果表明:1)与CON组相比,LB组7日龄仔猪空肠黏膜闭锁小带蛋白-1(ZO-1)基因相对表达量有提高趋势(P=0.08),而回肠黏膜ZO-1基因相对表达量则显著提高(P<0.05);LB组和HB组21日龄仔猪空肠和回肠黏膜ZO-1基因相对表达量均无显著变化(P>0.05)。2)与CON组相比,仔猪7日龄时,LB组空肠黏膜白细胞介素-8(IL-8)基因相对表达量有降低趋势(P=0.052),白细胞介素-10(IL-10)基因相对表达量呈提高趋势(P=0.063),但肿瘤坏死因子-α(TNF-α)和转化生长因子-β(TGF-β)基因相对表达量均无显著变化(P>0.05);HB组空肠黏膜IL-8基因相对表达量显著降低(P<0.05),但其他细胞因子基因相对表达量均无显著变化(P>0.05);LB组和HB组回肠黏膜IL-8、IL-10、TNF-α和TGF-β基因相对表达量均无显著变化(P>0.05)。与CON组相比,仔猪21日龄时,LB组空肠黏膜IL-8基因相对表达量显著降低(P<0.05),回肠黏膜IL-8基因相对表达量也显著降低(P<0.05),且IL-10和TGF-β基因相对表达量显著提高(P<0.05);HB组空肠黏膜IL-8基因相对表达量显著降低(P<0.05),TGF-β基因相对表达量显著提高(P<0.05),但回肠黏膜的4种细胞因子基因相对表�
The objective of this study was to compare the effects of live and heat-inactivated bacteria of Enterococcus faecium on expression of small intestinal tight junction proteins,cytokine responses,and Toll-like receptor(TLR)genes of piglets.Nice litters(10 to 11 piglets per litter)of healthy neonatal piglets(Duroc×Landrace×Yorkshire)were selected and randomly divided into three groups:control group(CON group),E.faecium group(LB group)and heat-inactivated E.faecium group(HB group),with 3 litters in each group.From birth to 6 days of age,piglets from 3 groups were orally fed sterile skimmed milk,skimmed milk containing E.faecium,and skimmed milk containing heat-inactivated E.faecium(concentration 1×10^(8) CFU/mL),10 mL/(head•d),respectively.Creep feed was added for piglets from 7 days of age to 21 days of age(weaned day).On 7 and 21 days of age,one piglet from each litter was selected for slaughter sampling to detect small intestine mucosa tight junction protein gene expression,cytokine gene expression and secretion,and gene expression of TLR.The results showed as follows:1)compared with the CON group,the relative expression level of zonula occludens-1(ZO-1)gene in jejunal mucosa of 7-day-old piglets in LB group had a trend to improve(P=0.08),while the ZO-1 gene relative expression level in ileal mucosa was significantly increased(P<0.05);There were no significant changes in relative expression level of ZO-1 gene in the jejunal and ileum of 21-day-old piglets in the LB and HB groups(P>0.05).2)At 7 days of age,the jejunal mucosal cytokine interleukin(IL)-8 gene relative expression level had a trend to decrease(P=0.052),and the IL-10 gene relative expression level had a trend to increase(P=0.063),however,neither tumor necrosis factor-alpha(TNF-α)nor transforming growth factor-beta(TGF-β)changed significantly(P>0.05).The relative expression level of IL-8 gene in the jejunal mucosa was significantly decreased in the HB group(P<0.05),however,there was no significant change in the relative expression of any other cyt
作者
崔百磊
王丽
郭乾鹏
黄怡
胡胜兰
CUI Bailei;WANG Li;GUO Qianpeng;HUANG Yi;HU Shenglan(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Guangdong Key Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture,State Key Laboratory of Livestock and Poultry Breeding,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2022年第12期7616-7627,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31460607)
广东省农业科学院十四五杰出团队项目(202106TD)。
