摘要
目的 实验观察新西兰白兔严重冻伤模型的大体、影像学、血液学、病理学变化。方法 采用乙醇浸泡法建立新西兰白兔严重冻伤模型12只,并进行常规复温治疗。分别于冻伤前、冻伤后复温前、复温后即刻、6 h、12 h、24 h、2 d、3 d、4 d、5 d、6 d对10只兔严重冻伤模型进行受累肢端肿胀程度、皮肤颜色观察及血管造影;于冻伤前、复温后即刻、6 h、12 h、24 h、2 d、3 d进行血常规、血生化、凝血及纤溶活性指标检测,放射免疫分析法测定血浆血栓素B2(TXB2)和6-酮-前列腺素F1α(6-keto-PGF1α)水平,TBA比色法测定血浆丙二醛(MDA)含量,黄嘌呤氧化酶法测定血浆超氧化物歧化酶(SOD)含量。于冻伤前、冻伤后复温前、复温后即刻和复温后6 h、12 h、24 h、2 d、3 d、4 d、5 d对其余2只模型受累肢端进行常规组织病理学检查。采用趾端截肢率和得分法计算模型的自然预后截肢率。结果 模型复温后即刻至复温后12 h,受累肢端皮肤颜色由紫蓝色转为粉红色,肿胀程度逐渐加重,肢端血管造影显示血流逐渐恢复,复温后12 h至截肢皮肤颜色由粉红色转变为紫蓝色,最后变为黑色,肿胀程度减轻至最后干瘪,肢端血管逐渐闭塞,血流逐渐消失,皮肤颜色紫蓝及暗黑区域大致对应血管闭塞区。白细胞水平在复温后即刻~3 d持续升高;红细胞、血红蛋白、红细胞比容于复温后即刻始升高,12 h达高峰,24 h恢复基线水平,2 d、3 d下降;血小板水平于复温后即刻~2 d持续下降,24 h达低谷,3 d恢复升高;丙氨酸转氨酶、天冬氨酸转氨酶、血尿素氮、血清肌酐值较基线值差异无统计学意义(P>0.05);6-keto-PGF1α水平于复温后即刻~12 h持续升高,12 h达高峰,24 h~3 d开始下降;TXB2水平于复温后即刻~3 d持续升高;MDA水平于复温后即刻~3 d持续升高;SOD水平于复温后即刻~24 h持续升高,24 h达高峰,2 d、3 d开始下降。模型左前肢血管病理检�
Objective To observe the gross, imaging, hematological, and pathological changes of severe frostbite in the New Zealand white rabbit models. Methods By using ethanol immersion method the severe frostbite model was established in 12 New Zealand rabbits, and conventional rewarming treatment was adopted. Observation of the affected limb swelling and skin color, and angiography were conducted in 10 rabbits before frostbite, between frostbite and rewarming, immediately after rewarming, as well as at 6 h, 12 h, 24 h,2 d, 3 d, 4 d, 5 d, 6 d after rewarming. Before frostbite, immediately after rewarming, and at 6 h, 12 h,24 h, 2 d, 3 d after rewarming, the blood routine, blood biochemistry, coagulation and fibrinolytic activity indicators were tested, the levels of plasma thromboxane B2(TXB2) and 6-keto-prostanin F1(6-keto-PGF1α)were determined by radioimmunoassay, the levels of plasma malondialdehyde(MDA) were determined by TBA colorimetry, and the superoxide dismutase(SOD) contents were determined by xanthine oxidase method. Before frostbite, between frostbite and rewarming, immediately after rewarming, as well as at 6 h, 12 h, 24 h, 2 d,3 d, 4 d, 5 d after rewarming, routine histopathological examinations of the affected limb toes were performed. The toe amputation rate and scoring method were used to calculate the natural prognostic amputation rate. Results Between immediately after rewarming and 12 h after rewarming, the color of the affected limb skin in severe frostbite models changed from purple to pink, while the swelling degree increased gradually. Angiography showed that the blood flow recovered gradually. During the period from the 12th hour after rewarming to amputation the skin color changed from pink to purple blue and finally to black, the swelling tissue was finally changed to the withered status, the limb blood vessels gradually occluded, the blood flow gradually disappeared, the skin areas presenting purple blue and black in color were roughly correspond to the vascular occlusion areas. The leukocyte
作者
付金鑫
段峰
张金龙
袁冰
张恒
阎洁羽
管阳
王燕
袁凯
王茂强
FU Jinxin;DUAN Feng;ZHANG Jinlong;YUAN Bing;ZHANG Heng;YAN Jieyu;GUAN Yang;WANG Yan;YUAN Kai;WANG Maoqiang(Department of Interventional Radiology,Chinese PLA General Hospital,Beijing 100853,China)
出处
《介入放射学杂志》
CSCD
北大核心
2022年第10期982-987,共6页
Journal of Interventional Radiology
关键词
冻伤
实验动物模型
血管造影
frostbite
experimental animal model
angiography
