摘要
探索橡实不同极性萃取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用。采用40%乙醇和纤维素酶组合提取,石油醚、乙酸乙酯依次萃取分离,获得橡实粗提物、石油醚相、乙酸乙酯相、萃余水相。通过测定酶抑制作用,研究各极性萃取物的体外降糖活性,并利用高效液相色谱(HPLC)和分子对接实验验证各提取物可能的活性成分。结果表明:橡实不同极性萃取物对α-淀粉酶和α-葡萄糖苷酶均有明显的抑制作用,其中乙酸乙酯相抑制效果最佳,其对α-淀粉酶和α-葡萄糖苷酶IC_(50)分别为1.590±0.073 mg/g、3.927±0.019(×10^(-3) mg/g);通过HPLC含量测定发现乙酸乙酯相中6种活性成含量最高,鞣花酸含量为500.75±6.93 mg/g,高于其他组分10~50倍;分子对接结果表明只有鞣花酸与α-淀粉酶和α-葡萄糖苷酶有良好的结合活性。以上结果表明,鞣花酸可能是橡实乙酸乙酯萃取物抑制α-淀粉酶和α-葡萄糖苷酶的主要活性成分。
To explore the inhibitory effects of different polar extracts of acorns onα-amylase andα-glucosidase.40%ethanol and cellulase were combined for extraction,followed by extraction and separation with petroleum ether and ethyl acetate to obtain crude acorn extract,petroleum ether phase,ethyl acetate phase and raffinate water phase.The in vitro hypoglycemic activity of each polar component was studied by measuring enzyme inhibition,and the possible active components of each extract were verified by high performance liquid chromatography(HPLC)and molecular docking assay.The results showed that the all extracts of different polarities of acorn had obvious inhibitory effects onα-amylase andα-glucosidase,and the ethyl acetate phase had the best inhibitory effect(IC_(50)=1.590±0.073 mg/g and 3.927±0.019(×10^(-3) mg/g),respectively).It was found by the HPLC content assay that the content of 6 active ingredients in the ethyl acetate phase was the highest,and the content of ellagic acid was 500.75±6.93 mg/g,which was 10-50 times higher than that of other components.Molecular docking results showed that only ellagic acid had good binding activity withα-amylase andα-glucosidase.The above results indicate that ellagic acid may be the main active component of acorn ethyl acetate extract to inhibitα-amylase andα-glucosidase.
作者
于聪聪
姚玉阳
耿雪营
苗启明
郭俊霞
张艳贞
米生权
YU Cong-cong;YAO Yu-yang;GENG Xue-ying;MIAO Qi-ming;GUO Jun-xia;ZHANG Yan-zhen;MI Sheng-quan(School of Health and Environment,Beijing Union University;Beijing Key Laboratory of Bioactive Substances and Functional Food,College of Biochemical Engineering,Beijing Union University,Beijing 100021,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第11期1838-1845,1899,共9页
Natural Product Research and Development
基金
北京联合大学人才强校优选计划(BPHR2017CZ03)。
