摘要
目的观察电针夹脊穴对脊髓损伤(SCI)大鼠血管内皮生长因子(VEGF)及其受体表达的影响,探讨其对SCI的修复作用及机制。方法60只雌性Wistar大鼠随机分为假手术组、模型组、夹脊电针组,各组再分为7 d和28 d两个亚组,每组10只。采用Allen’s改良式重物坠落法建立SCI大鼠模型,夹脊电针组在造模成功后给予夹脊电针治疗,于治疗7 d和28 d后应用BBB评分量表评定大鼠后肢功能,酶联接免疫吸附法(ELISA)检测大鼠血清中碱性成纤维细胞生长因子(bFGF)和血管生成素-1(Ang-1)水平变化,免疫组织化学法检测脊髓组织中VEGF的蛋白表达,蛋白质免疫印迹(Western blot)及反转录酶聚合酶链式反应(RT-PCR)法检测脊髓组织中VEGF及其受体VEGFR-2蛋白和mRNA的表达。结果与假手术组比较,术后7 d和28 d模型组和夹脊电针组大鼠BBB评分显著降低(P<0.01);血清中b FGF水平显著升高(P<0.01),Ang-1的水平显著降低(P<0.01);脊髓组织中VEGF的阳性细胞表达数明显增多(P<0.01),VEGF及VEGFR-2的蛋白和m RNA均显著增多(P<0.05,P<0.01);与模型组比较,夹脊电针组治疗7 d和28 d后,BBB评分显著升高(P<0.01),大鼠血清bFGF和Ang-1水平显著升高(P<0.01),同时脊髓组织VEGF的阳性细胞数及VEGF、VEGFR-2的蛋白和m RNA水平显著增加(P<0.01)。结论夹脊电针治疗能够有效恢复SCI大鼠运动能力,其作用机制可能与调控VEGF及其受体的表达,进而改善损伤组织微环境,促进血管生成相关。
Objective To observe the impact of electroacupuncture at Jiaji(EX-B2)on vascular endothelial growth factor(VEGF)and its receptor in rats with spinal cord injury(SCI)and discuss its role and mechanism in repairing SCI.Method Sixty female Wistar rats were randomized into a sham operation group,a model group,and a Jiaji electroacupuncture group;each group was further divided into 7 d and 28 d subgroups,with 10 rats in each group.The modified Allen’s weight-drop method was used to induce SCI rat models.The Jiaji electroacupuncture group received electroacupuncture at Jiaji.The Basso,Beattie,and Bresnahan(BBB)locomotor rating scale was adopted to estimate rat’s hind-limb function on day 7 and day 28;the enzyme-linked immunosorbent assay(ELISA)was used to detect the serum levels of basic fibroblast growth factor(bFGF)and angiogenin-1(Ang-1);the protein expression of VEGF in spinal cord tissues was examined using the immunohistochemistry technique;the protein and mRNA expression levels of VEGF and VEGF rector-2(VEGFR-2)were tested using Western blot and real-time polymerase chain reaction(RT-PCT)methods.Result Compared with the sham operation group,the BBB score dropped significantly in the 7 d and 28 d model and Jiaji electroacupuncture groups(P<0.01);the serum bFGF level rose notably(P<0.01),and the Ang-1 level declined markedly(P<0.01);the positive cell number of VEGF increased notably(P<0.01),and the protein and mRNA expression of VEGF and VEGFR-2 also increased substantially(P<0.05,P<0.01).Compared with the model group,the BBB score elevated significantly after 7 d or 28 d of treatments in the Jiaji electroacupuncture group(P<0.01);the serum bFGF and Ang-1 levels increased noticeably(P<0.01);the positive VEGF cells and the protein and mRNA expression levels of VEGF and VEGFR-2 also increased significantly in the spinal cord tissues(P<0.01).Conclusion Electroacupuncture at Jiaji can effectively improve the motor function of SCI rats.The mechanism may be associated with regulating the expression of VEGF and its r
作者
薛玉满
滕秀英
刘洋
许文婷
陆丽娜
时国臣
XUE Yuman;TENG Xiuying;LIU Yang;XU Wenting;LU Lina;SHI Guochen(Heilongjiang University of Chinese Medicine,Harbin 150001,China;Second Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150000,China;First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《上海针灸杂志》
2022年第10期1021-1027,共7页
Shanghai Journal of Acupuncture and Moxibustion
基金
黑龙江省中医药科研项目(ZHY2020-162)
黑龙江中医药大学科研基金项目(201730)
黑龙江中医药大学科研基金项目(2019MS24)
黑龙江省第二批省级名中医师承项目(2021-2023)。
关键词
电针
穴
夹脊
脊髓损伤
血管内皮生长因子
大鼠
Electroacupuncture
Point
Jiaji(EX-B2)
Spinal cord injury
Vascular endothelial growth factor
Rats
