摘要
目的:探讨血清高迁移率族蛋白B1(HMGB1)与糖尿病肾病(DN)病人肾功能、炎症因子及氧化应激指标的相关性。方法:117例DN病人根据24 h尿白蛋白定量(24hUPQ)分为微量白蛋白尿组(n=55例)和大量白蛋白尿组(n=62例),同期选择50例单纯2型糖尿病病人作为对照组。检测各组肾功能指标[血清肌酐(SCr)、尿素氮(BUN)、肾小球滤过率(GFR)、胱抑素C(Cys-C)]、炎症因子[血清核因子κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、细胞间黏附分子-1(ICAM-1)、白细胞介素-6(IL-6)]及氧化应激指标[丙二醛(MDA)、晚期氧化蛋白产物(AOPP)、超氧化物歧化醇(SOD)总抗氧化能力(T-AOC)],并分析血清HMGB1与肾功能、炎症因子及氧化应激指标的相关性。结果:大量白蛋白尿组和微量白蛋白尿组病人血清HMGB1表达水平均高于单纯2型糖尿病组,差异有统计学意义(P<0.05),而大量白蛋白尿组病人血清HMGB1表达水平高于微量白蛋白尿组,差异有统计学意义(P<0.05);各组病人SCr、BUN、GFR、Cys-C等肾功能指标比较差异均有统计学意义(P<0.01),单纯2型糖尿病组、微量白蛋白尿组、大量白蛋白尿组病人SCr、BUN、Cys-C依次逐渐升高,而GFR依次逐渐降低(P<0.01);各组病人血清NF-κB、TNF-α、ICAM-1、IL-6等炎症因子表达水平比较差异均有统计学意义(P<0.01),单纯2型糖尿病组、微量白蛋白尿组、大量白蛋白尿组病人血清NF-κB、TNF-α、ICAM-1、IL-6水平依次逐渐升高(P<0.01);各组病人血清MDA、T-AOC、SOD、AOPP等氧化应激指标比较差异均有统计学意义(P<0.01),单纯2型糖尿病组、微量白蛋白尿组、大量白蛋白尿组病人血清MDA、AOPP水平依次逐渐升高,而血清T-AOC、SOD水平依次逐渐降低(P<0.01);血清HMGB1与肾功能、炎症因子及氧化应激指标呈明显的相关性关系(P<0.05)。结论:DN病人血清HMGB1表达水平随病情严重程度加重而明显升高,与肾功能损伤、炎症及氧化应激反应严重�
Objective:To observe the correlation of serum high mobility group protein B1(HMGB1) with renal function,inflammatory factors,oxidative stress of patients with diabetic nephropathy(DN).Methods:One hundred and seventeen patients with DN were divided into the microalbuminuria group(n=55) and the large albuminuria group(n=62) according to the 24 hUPQ,and 50 patients with simple type 2 diabetes mellitus were selected as the control group during the same period.Renal function indexes [serum creatinine(SCr),blood urea nitrogen(BUN),glomerlar filtration rate(GFR),cystatin C(Cys-C)],inflammatory factors[nuclear factor-κB(NF-κB),tumor necrosis factor-α(TNF-α),intercellular cell adhesion molecule-1(ICAM-1),interleukin 6(IL-6)] and oxidative stress indicators[malondialdehyde(MDA),total antioxygen capability(T-AOC),superoxide dismutase(SOD),advanced oxidation protein products(AOPP)] in each group were measured,and the correlation of serum HMGB1 with renal function,inflammatory factors,oxidative stress indexes were analyzed.Results:The serum HMGB1 level in the large albuminuria group and the microalbuminuria group were higher than that in the simple type 2 diabetes mellitus group(P<0.05),while the serum HMGB1 level of patients in the large albuminuria group was higher than that in the microalbuminuria group(P<0.05).There was a statistically significant difference in three groups between the renal indicators such as SCr,BUN,GFR,Cys-C(P<0.01),and the SCr,BUN,Cys-C in the simple type 2 diabetes mellitus group,the microalbuminuria group and the large albuminuria group gradually increased,but the GFR gradually decrease(P<0.01).There was a statistically significant difference in the levels of serum NF-κB,TNF-α,ICAM-1,IL-6 in three groups(P<0.01).The levels of NF-κB,TNF-α,ICAM-1 and IL-6 in the simple type 2 diabetes mellitus group,the microalbuminuria group and the large albuminuria group gradually increased(P<0.01).There was a statistically significant difference in three groups between the oxidative stress indicators such
作者
黄丹
胡韬韬
陈丹
HUANG Dan;HU Tao-tao;CHEN Dan(Department of Nephrology,The Hospital of Chinese Integrated Traditional and Western Medicine of Wuhan,Wuhan Hubei 430000,China)
出处
《蚌埠医学院学报》
CAS
2022年第8期1021-1025,共5页
Journal of Bengbu Medical College
基金
湖北省卫生计生委中医药科研项目(ZY2019Q032)。
关键词
糖尿病肾病
高迁移率族蛋白B1
肾功能
炎症因子
氧化应激反应
diabetic nephropathy
high mobility group protein B1
renal function
inflammatory factors
oxidative stress response
