摘要
目的:探讨樱桃花色苷(SWCN)的降糖活性,为樱桃高价值深加工与开发提供理论依据。方法:用高脂喂养雄性C57BL/6J小鼠建立肥胖小鼠模型,测定小鼠血清中葡萄糖、胰岛素含量,采用口服葡萄糖耐量实验(OGTT)和胰岛素耐量实验(ITT),评价膳食SWCN对肥胖引起的葡萄糖不耐受和胰岛素抵抗的改善作用。用实时荧光定量PCR检测SWCN对肝脏中糖代谢相关基因的差异表达水平的影响。结果:膳食SWCN能够减缓肥胖的发生,降低肥胖小鼠的血糖、胰岛素水平,减轻胰岛素抵抗和葡萄糖不耐受性。实时荧光定量PCR实验结果表明,膳食SWCN能够提高肥胖小鼠肝脏胰岛素受体底物2(IRS2)的表达水平,同时下调糖异生途径的关键酶磷酸烯醇丙酮酸羧化激酶(PEPCK)和葡萄糖-6-磷酸酶(G6PC)基因的表达水平。结论:膳食樱桃花色苷能够激活肝脏中的胰岛素信号通路,抑制糖异生途径,从而降低肥胖小鼠的空腹血糖及胰岛素含量水平,增强糖耐量和胰岛素敏感性,改善胰岛素抵抗状态。
Objectives: This study aimed to investigate the hypoglycemic activity of sweet cherry(Prunus avium L.) anthocyanins(SWCN), thus providing a scientific basis for the promotion and application of sweet cherries as a functional agriculture product. Methods: Male C57 BL/6 J mice were fed with high-fat diet supplemented with SWCN. The glucose intolerance-improving effect of SWCN were evaluated based on an oral glucose tolerance test(OGTT), an insulin tolerance test(ITT) and the serum concentrations of glucose and insulin. Total liver RNA was extracted and real-time quantitative PCR was applied to determine the differential expression levels of genes related to glucose metabolism in the liver. Results: Mice fed HFD developed obesity, and dietary SWCN could decrease serum levels of glucose and insulin,and attenuated HFD-induced insulin resistance and glucose intolerance. Realtime PCR results showed that dietary SWCN significantly upregulated the transcription level of insulin receptor substrate-2(IRS2), and downregulated the transcription levels of phosphoenolpyruvate carboxykinase(PEPCK) and glucose-6-phsophatase catalytic(G6 PC). Conclusion: Dietary SWCN could activate the insulin signaling pathway and inhibit the gluconeogenesis pathway in the liver, thereby reducing the fasting blood glucose and insulin levels and enhancing glucose tolerance and insulin sensitivity in obese mice.
作者
宋海昭
沈新春
吴涛
郑晓冬
Song Haizhao;Shen Xinchun;Wu Tao;Zheng Xiaodong(College of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing 210023;College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2022年第4期117-124,共8页
Journal of Chinese Institute Of Food Science and Technology
关键词
樱桃花色苷
肥胖
胰岛素抵抗
糖异生
sweet cherry anthocyanins
obesity
insulin resistance
gluconeogenesis
