摘要
目的分析肥胖症内脏脂肪组织中环状RNA(circRNA)表达水平。方法留取北京朝阳医院2020年1月至9月收治患者的内脏脂肪组织,根据体重指数,分肥胖(>32.5 kg/m^(2))和正常组(<20.0 kg/m^(2)),选取10份(肥胖组6份,正常组4份)行全转录组测序,比较circRNA表达水平并利用生物信息学方法分析其功能。以Log2(Fold change>1)且P<0.05筛选差异化表达的circRNA,以P<0.05为条件筛选富集的通路和分子功能。结果与正常组比较,肥胖组有52个差异表达的circRNA[Log2(Fold change)>1,且P<0.05],其宿主基因主要富集在"胰岛素抵抗"、"肿瘤坏死因子通路"和"细胞因子-因子受体通路"等通路(P<0.05),筛选出上述通路中的关键基因,如趋化因子(CX3CL1)、丝裂原活化蛋白激酶(MAPK)、白细胞介素6(IL-6)、胰岛素受体底物2(ISR2)等,这些基因与慢性炎性反应密切相关。结论circRNAs可能通过调控慢性炎性反应在肥胖症发生发展中发挥作用。
Objective To compare expression profiles of circular RNA(circRNA)in visceral adipose tissue(VAT)of obesity[body mass index(BMI)>32.5 kg/m^(2)]and lean(BMI<20.0 kg/m^(2))individuals.Methods The whole transcriptome RNA sequencing was performed to detect the expression of circRNAs,and bioinformatic methods were used for function analysis,circRNAs with a Log2(fold change)>1 and P value of<0.05 were considered as dysregulated,and the enriched pathways with a P value of<0.05 were selected for further analysis.Results A total of 52 circRNAs were differentially expressed in VAT between obesity and lean individuals,including 25 up-and 27 down-regulated circRNAs[Log2(fold change)>1,P<0.05].Pathway enrichment analysis indicated that those circRNAs were mainly involved in insulin resistance,TNF pathway,cytokine-cytokine receptor interaction(P<0.05).A further analysis selected hub genes enriched in those Kyoto gene and genome encyclopedia(KEGG)pathways,which were mitogen activated kinase-like protein(MAPK),interleukin 6(IL-6),insulin receptor substrate 2(ISR2),all these genes were associated with chronic inflammation.Conclusion The circRNA might play an important role in the pathogenesis of obesity by regulating inflammation.
作者
李干斌
杨磊
张皓宇
曹可
翟志伟
王振军
韩加刚
Li Ganbin;Yang Lei;Zhang Haoyu;Cao Ke;Zhai Zhiwei;Wang Zhenjun;Han Jiagang(Department of General Surgery,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China;Medical Research Center of Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China)
出处
《中华实验外科杂志》
CAS
北大核心
2022年第3期552-555,共4页
Chinese Journal of Experimental Surgery
