摘要
目的观察樱桃花青素苷对佐剂性关节炎大鼠T淋巴细胞亚群、脾细胞增殖及血清IL-6和足爪PGE2水平的影响。方法将60只SD雄性大鼠随机分为6组,即正常组、模型组、樱桃花青素苷高、中、低剂量(300、150、75mg/kg)组及雷公藤多苷(8.75mg/kg)治疗组(简称雷公藤组),除正常组外,用弗氏完全佐剂制备模型。流式细胞术检测大鼠外周全血中CD3、CD4、CD8T细胞亚群和CD4/CD8值;光镜下观察踝关节组织形态学改变;MTT法检测脾细胞增殖能力;放免法检测血清IL-6水平与足爪匀浆中PGE2水平。结果与正常组相比,模型组外周全血中CD3、CD4T细胞下降(P<0.01),樱桃花青素苷和雷公藤组外周全血中CD3、CD4T细胞升高。光镜下组织形态观察表明花青素苷高剂量组和雷公藤组能减轻滑膜增生,减少炎性细胞浸润,减轻组织损伤。在ConA刺激下模型组大鼠脾细胞增殖反应与正常组相比降低,樱桃花青素苷高剂量能使模型大鼠低下的脾细胞增殖反应恢复至接近正常。模型组血清IL-6水平高于正常组,樱桃花青素苷各剂量组血清IL-6明显降低(P<0.01);模型组足爪PGE2水平高于正常组(P<0.01),樱桃花青素苷高剂量组和雷公藤组PGE2水平比模型组降低(P<0.01)。结论樱桃花青素苷高剂量对佐剂性关节炎大鼠模型的T淋巴细胞亚群和免疫功能有调节作用,并能显著降低炎症细胞因子IL-6和足爪PGE2水平,从而减轻模型大鼠的关节炎损伤。
Objective To assess the potential immunological activity of anthocyanins from cherries on adjuvant arthritis (AA) rats and observe the effect on the ratio of T lymphocyte subpopulation and splenic lymphocyte and the levels of IL-6 in serum and PGE_2 in paws. Methods Sixty male Spraque Dauley rats were randomly allocated to six groups, they were normal group, model group, anthocyanins (300, 150, 75 mg/kg) -treated groups, and polyglycosides of Tripterygium wilfordii (TW)-treated group. Arthritis was induced by the complete Freunds adjuvant. The T lymphocyte subpopulation of CD3, CD4, and CD8 and CD4/CD8 value in peripheral blood were assayed by FACA Calibur flow cytometer. The splenocyte proliferation was assessed by means of MTT Assays. IL-6 levels in serum and PGE_2 in paws were determined by radioimmunoassay. Results The morphological changes in the hind limbs were conducted under a light microscope. CD3 and CD4 T cells were found decreased (P<0.01) in model group, while increased in anthocyanin and TW-treated groups in AA rats vs normal group. Histological examination of sections through the hind limbs revealed the alleviation of synovial hyperplasia, inflammatory reaction, and tissue damage in the joint after the treatment with anthocyanins at high dosage group and in TW-treated group. The anthocyanins at high dosage could improve the splenocyte proliferation induced by Con-A stimulation to normal. Both of the IL-6 and PGE_2 levels in model group were higher than that in normal group. IL-6 levels in serum were decreased in anthocyanin groups (P<0.01) and PGE_2 in paws were decreased (P<0.01) at anthocyanins high dosage and polyglycosides of TW-treated group. Conclusion The anthocyanins could alleviate the inflammatory reaction in the joint, the mechanism might be via the increase of the CD3 and CD4 of T cell subpopulation in peripheral blood, the improvement of the splenocyte proliferation and the decrease of the IL-6 level in serum and PGE_2 in paw. The results suggest that the anthocyanins could be one o
出处
《中草药》
CAS
CSCD
北大核心
2005年第6期874-878,共5页
Chinese Traditional and Herbal Drugs
基金
科技部"863"计划创新药物和中药现代化重大专项资助(2002AA2Z3231)
