摘要
为挖掘高粒重小麦种质资源,连续2年在宁夏引黄灌区生态条件下测定了209份小麦种质资源的千粒重、粒长、粒宽和粒厚4个籽粒相关性状,同时用4个粒重相关基因TaGW2-6B、TaGASR、TaGS-D1和TaCWI-4A的KASP标记对参试材料进行基因型检测。结果表明,宁夏小麦种质资源籽粒表型性状较丰富。千粒重、粒长、粒宽和粒厚分布范围分别为29.73~56.25 g、5.82~7.57 mm、2.87~3.83 mm和2.74~3.55 mm。4个基因标记分别将参试材料区分为两种单倍型:TaGW2-6B将参试材料区分为Hap-6B-1和Others两种单倍型,其中优异单倍型Hap-6B-1的频率为62.44%;TaGASR将参试材料区分为H1c和H1c/H1g两种单倍型,其中优异单倍型H1c频率为93.10%;TaCWI-4A-1523将参试材料区分为Hap-4A-C和Hap-4A-T两种单倍型,其中优异单倍型Hap-4A-C频率为76.41%;TaGS-D1将参试材料区分为Ta GS-D1a和TaGS-D1b两种单倍型,其中优异单倍型TaGS-D1a频率为86.50%。TaGW2-6B与千粒重显著相关,与粒宽和粒厚极显著相关;TaGASR与千粒重和粒长极显著相关,与粒宽显著相关;TaCWI-4A与千粒重、粒宽和粒厚显著相关。宁夏小麦种质资源粒重基因主要包括7种优异单倍型组合,其中,Hap-6B-1+H1c+Hap-4A-C+TaGS-D1a组合对千粒重、粒宽和粒厚有显著的正向调控作用,Hap-4AC+TaGS-D1a组合对粒长有显著的正向调控作用。因此,在宁夏引黄灌区生态条件下TaGW2-6B、TaGASR和TaCWI-4A能够较好地区分小麦粒重大小,可用于粒重性状选择。在209份参试材料中,共筛选到14份千粒重大于50 g的高粒重材料,其中有9份材料聚合了4个粒重相关基因的优异单倍型。
To analyze the yield potential of common wheat(Triticum aestivum L.)using high-grain weight germplasm resources in Ningxia,here we conducted the field trials for detecting grain weight under the ecological conditions of the Yellow River irrigation area,as well as marker-assisted analysis using four functional KASP markers TaGW2-6B,TaGASR,TaGS-D1 and TaCWI-4A. Four grain-related traits consisting of the thousandkernel weight(TKW),kernel length(KL),kernel width(KW)and kernel thickness(KT)of 209 wheat germplasm resources were measured for 2 consecutive years. The results showed that wheat germplasm resources had abundant diversity on grain-related traits. The distribution ranges of TKW,KL,KW and KT values were 29.73-56.25 g,5.82-7.57 mm,2.87-3.83 mm and 2.74-3.55 mm,respectively. For TaGW2-6B,two haplotypes(Hap-6B-1 and Others)were detected,while the elite haplotype Hap-6B-1 accounted for 62.44%. For TaGASR two haplotypes(H1c and H1c/H1g)were detected,while haplotype H1c accounted for 93.10%. Two haplotypes Hap-4A-C and Hap-4A-T at TaCWI-4A-1523 were revealed,and the elite haplotype Hap-4A-C accounted for 76.41%. Two haplotypes TaGS-D1a and TaGS-D1b at the TaGS-D1 were revealed,and the elite haplotype TaGS-D1a accounted for 86.50%. The locus TaGW2-6B was found to be significantly correlated with TKW and extremely significantly correlated with KW and KT. TaGASR was found to be significantly correlated with TKW,KL and KW. TaCWI-4Awas found to be significantly correlated with TKW,KW and KT. Based on the genotyping results,seven haplotype combinations were revealed. The combination of Hap-6B-1+H1c+Hap-4A-C+TaGSD1a represented a significant positive effect on TKW,KW and KT,while the combination of Hap-4A-C+TaGSD1a represented a significant positive effect on KL. Taken together,three genes TaGW2-6B,TaGASR and TaCWI-4Amight become elite makers on selection of the wheat grain weight under the ecological conditions of the Yellow River irrigation area in Ningxia. Out of 209 tested genotypes,14 high-grain weight genotypes(1000
作者
张维军
赵俊杰
何进尚
郝晨阳
王小亮
亢玲
张富国
张学勇
陈东升
ZHANG Wei-jun;ZHAO Jun-jie;HE Jin-shang;HAO Chen-yang;WANG Xiao-ling;KANG Ling;ZHANG Fu-guo;ZHANG Xue-yong;CHEN Dong-shen(The Crop Research Institute,Ningxia Academy of Agriculture and Forestry Science,Yinchuan 750002;Institute of Cotton Sciences,Chinese Academy of Agricultural Sciences,Henan Anyang 455000;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;Ningxia Academy of Agriculture and Forestry Science,Yinchuan 750002)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2022年第2期493-504,共12页
Journal of Plant Genetic Resources
基金
宁夏自然科学基金项目(2021AAC03275)
中央引导地方科技发展专项(2020YDDF0056)
宁夏农业育种专项(2018NYYZ02)
宁夏农业科技自主创新专项(NKYJ-20-05)。
