摘要
小麦耐热性是多基因控制的复杂数量性状,通过关联耐热性状获得耐热分子标记,利用该标记辅助选择育种可提高育种的准确性,加快小麦耐热育种进程。本研究对187份小麦品种(系)的千粒重、籽粒长度、籽粒宽度、籽粒表面积和籽粒周长5个籽粒相关性状的热感指数进行关联分析,鉴定出2个耐热相关分子标记Xwmc622、Xwmc707。聚丙烯酰胺凝胶电泳分析表明,Xwmc622、Xwmc707各自具有Ⅰ、Ⅱ、Ⅲ3个等位变异类型。Xwmc622的Ⅰ型等位位点与千粒重热感指数、籽粒宽度热感指数和籽粒周长热感指数呈极显著负相关关系,同时Ⅰ型等位位点与籽粒长度热感指数和籽粒表面积热感指数呈显著负相关关系,Ⅰ型小麦品种(系)的热感指数平均值小于1;Ⅱ型等位位点与千粒重热感指数呈极显著正相关关系,与籽粒长度热感指数、籽粒表面积热感指数、籽粒周长热感指数呈显著正相关关系,Ⅱ型等位变异小麦品种(系)的热感指数平均值大于1;Ⅲ型等位变异与小麦籽粒性状热感指数相关性不显著。Xwmc707的Ⅰ型等位变异与小麦籽粒性状热感指数相关性不显著;Ⅱ型等位位点与千粒重热感指数呈显著负相关关系,Ⅱ型小麦品种(系)的热感指数平均值小于1;类型Ⅲ与小麦材料的千粒重热感指数、籽粒长度热感指数、籽粒周长热感指数呈显著正相关,Ⅲ型等位位点小麦品种(系)的热感指数平均值大于1。研究结果表明:Xwmc622的Ⅰ型等位位点为耐热优良等位变异;Xwmc707的Ⅱ型等位位点为优良等位变异。这为小麦耐热分子标记辅助育种提供了参考信息。
Heat tolerance of wheat is a complex quantitative trait controlled by multiple genes.Molecular markers related to heat tolerance can be acquired by correlation analysis.With these tolerant markers,the accuracy of molecular marker-assisted selection breeding may be improved,and the process of breeding for heat tolerant wheat may be accelerated.In this study,heat sensitivity indexes(HI)of five grain traits,including 1000-kernel weight,kernel length,kernel width,kernel surface area,and kernel circumference were identified in 187 wheat varieties(lines).Molecular markers Xwmc622 and Xwmc707 related to heat sensitivity indexes were obtained by simple correlation analysis.Polyacrylamide gel electrophoresis analysis showed that Xwmc622 and Xwmc707 each had three allelic types:Ⅰ,ⅡandⅢ.TypeⅠallelic loci of Xwmc622 were extremely significantly negatively correlated with HI of 1000-kernel weight,of kernel width,and of kernel circumference,and significantly negatively correlated with HI of kernel length and of kernel surface area.The average values of HI in wheat varieties(lines)with typeⅠallelic variation were less than 1.TypeⅡallelic loci of Xwmc622 were extremely significantly positively correlated with HI of 1000-kernel weight,and significantly positively correlated with HI of kernel length,of kernel surface area,and of kernel circumference.The average values of HI in wheat varieties(lines)with typeⅡallelic variation were greater than 1.TypeⅢallelic loci of Xwmc622 were not significantly correlated with HI of grain traits.TypeⅠallelic loci of Xwmc707 were not significantly correlated with HI of wheat grain traits.TypeⅡallelic loci of Xwmc707 were significantly negatively correlated with HI of 1000-kernel.The average values of HI in wheat varieties(lines)with typeⅡallelic variation were less than 1.TypeⅢallelic loci of Xwmc707 were significantly positively correlated with HI of 1000-kernel weight,of kernel length,and of kernel circumference.The average values of HI of the grain traits in wheat varie
作者
邵雪飞
李国良
杨阳
付才
李孟军
赵敏
刘子会
SHAO Xue-fei;LI Guo-liang;YANG Yang;FU Cai;LI Meng-jun;ZHAO Min;LIU Zi-hui(College of Landscape architecture and Ecological Engineering,Hebei University of Engineering,Handan 056000;Institute of Biotechnology and Food Science,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051;Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050000)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2021年第6期1635-1643,共9页
Journal of Plant Genetic Resources
基金
河北省现代农业创新工程(494-0402-YBN-S2XB,494-0402-YBN-CTGQ)
河北省重点研发计划项目(21326322D)
石家庄市科学技术研究与发展规划(191490332A)。
关键词
小麦
等位变异
分子标记
耐热性
wheat
allelic variation
molecular markers
heat tolerance
