摘要
为探究不同杜梨资源对盐碱胁迫的生理响应及筛选耐盐碱资源,以甘肃、山西、辽宁等地收集的17份杜梨资源3000余株幼苗为试验材料,采用中性盐和碱性盐混合胁迫处理,测定盐碱抗性相关的生理指标,基于主成分分析法、热图及聚类分析法和抗逆系数法进行抗性评价。研究发现,抗性强的资源抗氧化酶活性和脯氨酸含量在受到胁迫后显著上升,300 mmol/L盐碱胁迫下超氧化物歧化酶活性显著上升的资源为河底杜梨3和平陆杜梨1,分别上升了75.04%、70.04%;过氧化物酶活性上升显著的资源为甘肃杜梨1和河底杜梨1,分别上升了1136.11%、742.86%;过氧化氢酶活性显著上升的资源为甘肃杜梨15、甘肃杜梨4,分别上升了344.58%、343.42%,脯氨酸含量显著上升的资源为平陆杜梨2、河底杜梨1、河底杜梨3,丙二醛含量上升较多的资源为甘肃杜梨16、甘肃杜梨13、甘肃杜梨1。除甘肃杜梨13在低浓度(100 mmol/L)盐碱胁迫即出现明显盐害现象外,其他杜梨资源均在300 mmol/L浓度处理下才开始出现不同程度的盐害;利用不同方法进行耐盐碱性分析结果存在一定的差异,其中基于主成分分析法发现河底杜梨1、河底杜梨3、平陆杜梨1、甘肃杜梨15、山西杜梨1耐盐碱性强;基于热图和聚类分析发现甘肃杜梨4、甘肃杜梨15、平陆杜梨1、河底杜梨1和河底杜梨3具有较强的耐盐碱性;而利用抗逆系数法分析耐盐碱性较强的资源为甘肃杜梨1、河底杜梨1、甘肃杜梨16、甘肃杜梨4、甘肃杜梨15。综合3种方法的分析结果,河底杜梨1和甘肃杜梨15属于高抗盐碱类型,而甘肃杜梨13则为盐碱敏感型资源。
To unlock the physiological responses of P. betulaefolia Bunge germplasm resources and identify the elite resources under saline-alkali stress conditions,>3000 seedling plants from 17 betulaefolia Bunge accessions in Gansu,Shanxi and Liaoning provinces were analyzed. The physiological indexes under the mixed stress treatments of neutral salt and alkaline salt were measured,followed by statistical analysis(principal component analysis,heat map,cluster analysis and stress coefficient). In P. betulaefolia Bunge plants showing strong tolerance,the antioxidant enzyme activity and proline content increased significantly after treatments.Under 300 mmol/L salt-alkali stress,the SOD activity in P. betulaefolia-Hedi 3 and P. betulaefolia-Pinglu 1was observed with increase up to 75.04% and 70.04%,respectively. The increase on the POD activity in P.betulaefolia-Gansu 1 and P. betulaefolia-Hedi 1 was detected up to 1136.11% and 742.86%,respectively. In P.betulaefolia-Gansu 15 and P. betulaefolia-Gansu 4 a significant increase(344.58% and 343.42%,respectively)on the CAT activity was detected. Moreover,the significant increase on proline content were observed in P.betulaefolia-Pinglu 2,P. betulaefolia-Hedi 1 and P. betulaefolia-Hedi 3 and MDA content were observed in P.betulaefolia-Gansu 16,P. betulaefolia-Gansu 13 and P. betulaefoli-Gansu 1. Except for P. betulaefolia-Gansu 13 that showed salt damage at low concentration(100 mmol/L)under saline-alkali stress,other P. betulaefolia Bunge resources showed degrees of salt damage under 300 mmol/L. By deployment of the principal component analysis,P. betulaefolia-Hedi 1,P.betulaefolia-Hedi 3,P. betulaefolia-Pinglu 1,P. betulaefolia-Gansu 15 and P.betulaefolia-Shanxi 1 showed strong salt-alkali resistance. Based on heat map and cluster analysis,it was found that P. betulaefolia-Gansu 4,P. betulaefolia-Gansu 15,P. betulaefolia-Pinglu 1,P. betulaefolia-Hedi 1 and P.betulaefolia-Hedi 3 had strong salinity tolerance. The coefficient analysis suggested that P. betulaefolia-Gansu 1,P. betu
作者
霍宏亮
王超
杨祥
曹玉芬
田路明
董星光
张莹
齐丹
徐家玉
刘超
HUO Hong-liang;WANG Chao;YANG Xiang;CAO Yu-fen;TIAN Lu-ming;DONG Xing-guang;ZHANG Ying;QI Dan;XU Jia-yu;LIU Chao(Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Liaoning Xingcheng 125100;College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2022年第2期480-492,共13页
Journal of Plant Genetic Resources
基金
中国农业科学院科技创新工程(CAAS-ASTIP)
财政部和农业农村部:国家现代农业产业技术体系(CARS-28-1)。
关键词
杜梨
盐碱胁迫
生理响应
综合评价
P.betulaefolia Bunge
salt-alkali stress
physiological response
comprehensive evaluation
