摘要
为促进耐盐碱大豆品种选育和盐碱地合理开发与利用,以4个黑龙江省大豆品种(红研12、垦科豆14、垦科豆28和垦豆62)为试材,以6个梯度(0,30,60,90,120和150 mmol·L^(-1))盐碱混合液(NaCl∶Na_(2)SO_(4)∶NaHCO_(3)∶Na_(2)CO_(3)摩尔比为1∶9∶9∶1)对试材进行盐碱胁迫处理,采用培养皿滤纸培养法进行发芽试验,分析盐碱胁迫对大豆种子萌发参数及胚根参数的影响。结果表明,低浓度盐碱胁迫(30 mmol·L^(-1))处理,对红研12萌发有一定促进作用,但对其他品种发芽率和发芽势有较小的抑制作用;随盐碱混合液浓度增大,各处理发芽势和发芽率受到抑制作用也逐渐增强,相同盐碱混合液浓度对不同品种发芽势和发芽率影响不同;盐碱胁迫浓度≥60 mmol·L^(-1)时,大豆种子发芽率和发芽势及耐盐碱指数下降;盐碱胁迫浓度为90 mmol·L^(-1)时,与CK相比,发芽势、发芽率、耐盐碱指数差异均达到显著水平。盐碱胁迫浓度为150 mmol·L^(-1)时,发芽指标受到的抑制作用最强。盐碱胁迫抑制胚根生长,随盐碱混合液浓度增大,胚根长度、胚根鲜重和干重抑制作用越强,不同品种受到抑制程度不同,与发芽势和发芽率相比,胚根长度、胚根鲜重和干重随盐碱胁迫程度增加受到抑制程度更大,下降幅度更大。在相同处理浓度下,不同大豆品种胚根干重受到抑制程度不同。盐碱混合液浓度为90 mmol·L^(-1)时,各处理的胚根参数指标与CK差异均达到显著水平。综合上述,盐碱胁迫浓度为90 mmol·L^(-1)可作为大豆萌发期耐盐碱鉴定处理液的适宜浓度。
In order to promote the breeding of salt alkali tolerant soybean varieties and the rational development and utilization of saline alkali land,four soybean varieties from Heilongjiang Province(Hongyan 12,Kenkedou 14,Kenkedou 28,Kendou 62)were used as test materials.Six gradients(0,30,60,90,120 and 150 mmol·L^(-1))of salt alkali mixed solution(NaCl∶Na_(2)SO_(4)∶NaHCO_(3)∶Na_(2)CO_(3)molar ratio of 1∶9∶9∶1)were used to treat the test materials with salt alkali stress.The germination experiment was conducted using filter paper culture in a culture dish to analyze the effects of salt alkali stress on soybean seed germination parameters and embryonic root parameters.The results showed that low concentration saline alkali stress(30 mmol·L^(-1))treatment had a certain promoting effect on the germination of Hongyan 12,but had a relatively small inhibitory effect on the germination rate and germination potential of other varieties.As the concentration of saline alkali mixture increases,the inhibitory effect on germination potential and germination rate of the treatment also gradually increases.The same concentration of saline alkali mixture has different effects on germination potential and germination rate of different varieties;When the concentration of salt alkali stress was≥60 mmol·L^(-1),the germination rate,germination potential,and salt alkali tolerance index of soybean seeds decrease;When the concentration of salt alkali stress was 90 mmol·L^(-1),compared with CK,the differences in germination potential,germination rate,and salt alkali tolerance index all reach significant levels.When the concentration of salt alkali stress was 150 mmol·L^(-1),the germination index was most inhibited and the embryonic root growth was inhibited.As the concentration of salt alkali mixture increases,the inhibitory effect on root length,root fresh weight,and dry weight became stronger.Different varieties were subject to different degrees of inhibition.Compared with germination potential and germination rate,embryonic
作者
苏文静
赵曼儒
丁小飞
李倩
魏玮
蒋红鑫
周培禄
宋晓慧
SU Wenjing;ZHAO Manru;DING Xiaofei;LI Qian;WEI Wei;JIANG Hongxin;ZHOU Peilu;SONG Xiaohui(Tianjin Agricultural College/Tianjin Key Laboratory of Intelligent Breeding of Major Crops,Tianjin 300392,China;Crop and Development Research Institute,Heilongjiang Agricultural Reclamation Sciences,Jiamusi 154007,China)
出处
《黑龙江农业科学》
2024年第5期13-18,共6页
Heilongjiang Agricultural Sciences
基金
国家十四五重点研发计划(2021YFD1201101)
天津市高等学校大学生创新创业训练计划(202310061149)。
关键词
大豆
盐碱胁迫
萌发参数
胚根参数
soybean
salinity stress
germination parameters
radicle parameters
