摘要
为评价马铃薯耐盐性,筛选耐盐种质资源,本研究以164份二倍体马铃薯杂种后代及其亲本为试验材料,进行NaCl胁迫处理,测量盐胁迫和对照条件下杂交后代和亲本的芽长、芽鲜重、芽干重、根长、根鲜重和根干重6个形态指标,然后运用隶属函数分析和聚类分析方法进行综合评价及分类。结果表明,以亲本为对照,比较综合评价D值,筛选出26个耐盐无性系和10个盐敏感无性系;利用6个形态指标和D值分别聚类分析,166个无性系均被分为4个耐盐性不同的类群,表明可以分别利用6个形态指标和D值进行聚类,筛选出在同一类群中共同包含的无性系,提高耐盐评价的精确性;后代无性系根长相对值平均值较高(62.5),且聚类分析后,第一类群和第二类群的根长相对值的差异较小,说明根长不是理想的形态筛选指标,可以考虑用主根数替代根长,作为耐盐筛选的形态指标之一。本研究结果为快速评价大量马铃薯种质资源的耐盐性和马铃薯耐盐育种提供了理论依据和材料基础。
The objectives of this research were to evaluate the salt tolerance of potato and to screen salt tolerant potato germplasm resource.One hundred and sixty-four diploid potato(Solanum phureja×S.stenotomum)hybrid progenies and two parents under 80 mmol·L-1 NaCl stress were assessed for six morphological indexes of shoot length,shoot fresh weight,shoot dry weight,root length,root fresh weight and root dry weight,and comprehensively evaluation and clustering using subordinate function analysis were conducted.Twenty-six salt tolerant and ten salt susceptible clones were identified by D values of progenies compared with those of parents.One hundred and sixty-six clones(parents)were separated into four clusters of different salt tolerance using cluster analysis of six morphological traits and D values.If no control was taken,six morphological and D values could be used to cluster and to screen the clones that are included in the same group,which will improve the accuracy of salt tolerance evaluation.The average relative value of root length of progenies was higher(62.5).After cluster analysis,the difference in relative values of the root length between the first and the second groups was also small,indicating that the root length is not an ideal morphological screening index,and the root number could be considered as a morphological index for salt tolerance.The results of this study provide a reference for rapid evaluation of salt tolerance of a large number of potato germplasm resources,and provide a material basis for potato salt tolerance breeding.
作者
赵明辉
白雅梅
吕文河
ZHAO Minghui;BAI Yamei;LYU Wenhe(College of Food Engineering,Harbin University,Harbin,Heilongjiang 150086;College of Agriculture,Northeast Agricultural University,Harbin,Heilongjiang 150030;College of Resources and Environment,Northeast Agricultural University,Harbin,Heilongjiang 150030)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第1期1-9,共9页
Journal of Nuclear Agricultural Sciences
基金
国家科技支撑计划课题(2012BAD06B02)
黑龙江省经济作物现代农业产业技术协同创新体系(HNWJZTX201701)
哈尔滨学院青年(博士)科研基金项目(HUDF2015-005).
关键词
马铃薯
二倍体
耐盐
评价
potato
diploid
salt tolerance
evaluation
