摘要
为研究中药材黑蛞蝓(Agriolimax agrestis)的化学成分及其化合物的α-糖苷酶抑制活性,本研究采用Sephadex LH-20、硅胶、Chromatorex C_(18)等柱色谱对黑蛞蝓正己烷提取物进行化学成分离纯化,通过NMR波谱数据与文献对比分析鉴定化合物结构。从黑蛞蝓正己烷提取物中共分离得到15个化合物,分别鉴定为苯乙醇(1)、十六烷酸(2)、(Z)9-octadecenoic acid(3)、1-(2-hydroxyethoxy)ethyl(E)-octadec-9-enoate(4)、二十七烷(5)、dodecyl(Z)-9-hexadecenoate(6)、cis,cis-diunsaturatedα-meromycolic acid(7)、1,2,3-propanetriyl(9 Z,9′Z,9″Z)tris(-9-octadecenoate)(8)、胆固醇(9)、7-酮基胆固醇(10)、胆甾-5-烯-3β,7α二醇(11)、胆甾-5-烯-3β,7β二醇(12)、胆甾醇肉豆蔻酸酯(13)、胆甾醇基十七酸酯(14)、熊果酸(15)。除化合物9外的所有化合物均为首次从黑蛞蝓中分离得到,α-糖苷酶抑制活性筛选结果显示7个化合物对α-糖苷酶的抑制活性均较弱。
To study the chemical constituents of Agriolimax agrestis and theα-glycosidase inhibitory activity of its compounds.The chemical constituents of hexane extract from Agriolimax agrestis were isolated and purified by column chromatography,such as Sephadex LH-20,silica gel,Chromatorex C_(18),etc.,and the structures of compounds identified by the NMR spectroscopic data analysis and comparison with literature.Fifteen compounds were obtained from the hexane extract of Agriolimax agrestis and their chemical structures identified as phenylethyl alcohol(1),palmitic acid(2),(Z)9-octadecenoic acid(3),1-(2-hydroxyethoxy)ethyl(E)-octadec-9-enoate(4),heptacosane(5),dodecyl(Z)-9-hexadecenoate(6),cis,cis-diunsaturatedα-meromycolic acid(7),1,2,3-propanetriyl(9 Z,9′Z,9″Z)tris(-9-octadecenoate)(8),cholesterol(9),7-ketocholesterol(10),5-ene-3β,7α-diol(11),5-ene-3β,7β-diol(12),cholesteryl myristate(13),cholesteryl hepadecanoate(14),and ursolic acid(15).All compounds were isolated from Agriolimax agrestis for the first time except for compound 9.The screening results ofα-glycosidase inhibitory activity show that 7 compounds have weak inhibitory activity onα-glycosidase.
作者
张祖湘
王亚凤
张瑛
何瑞杰
韦用琼
阳丙媛
阮俊
黄永林
ZHANG Zu-xiang;WANG Ya-feng;ZHANG Ying;HE Rui-jie;WEI Yong-qiong;YANG Bing-yuan;RUAN Jun;HUANG Yong-lin(Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization,Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China;Guangxi Jiufu Biotechnology Co.,Ltd.,Nanning 530012,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第1期63-69,共7页
Natural Product Research and Development
基金
国家自然科学基金(82060764)
广西自然科学基金(2018GXNSFAA281078)
广东省重点领域研发计划资助项目(2020B1111110003)
中央引导地方科技发展专项(桂科ZY20111010)。
关键词
黑蛞蝓
化学成分
结构鉴定
α-糖苷酶抑制活性
Agriolimax agrestis
chemical constituents
structure identification
α-glycosidase inhibitory activity
