摘要
目的运用网络药理学研究方法探讨人参,红参及黑参抗肺气虚的作用机制与物质基础。方法①通过TCMSP并结合相关文献筛选人参,红参及黑参的化学成分和靶点;②采用人类基因数据库Gene Cards获取慢性支气管炎的相关靶点,再将疾病靶点分别与人参、红参及黑参的靶点取交集;③得到各药物和疾病的共同靶点后在STRING数据库中进行蛋白相互作用分析,利用Cytoscape软件建立药物-成分-疾病-靶点互作网络得到治疗疾病的主要有效成分;④运用计算机R语言分析技术进行GO和KEGG富集分析。结果利用TCMSP及相关文献筛选出各药物的有效成分后根据药物-成分-疾病-靶点互作网络Degree值进行排序,人参中前10种成分为人参皂苷Rh4、Rg5、山柰酚、人参皂苷Rh1、Ra2、三七皂苷R2、三七皂苷R6、人参皂苷Ra0、Ra1、Ra3;红参中前10种成分为人参皂苷Rh4、Rh1、三七皂苷R2、人参皂苷Rg2、Rb1、Rb2、Rc、Re、Rs1、β-谷甾醇;黑参前10种成分为人参皂苷Rh3、Rh4、Rk3、Rk1、Rg5、F4、20(S)-Rh2、20(R)-Rh2、Rh1、Rg3。人参、红参及黑参治疗慢性支气管炎潜在靶点分别为46、39、53个。根据Degree>15筛选得到除三者共同的靶蛋白MAPK1、MAPK8、STAT3、VEGFA、MTOR、MMP9外,还有人参的CASP3,红参的CASP8、BCL2L1,黑参的AKT1、MMP2及MAPK14。通过GO功能和KEGG通路的富集分析,根据P值分别筛选人参、红参及黑参前20个GO条目和前10个KEGG富集通路。三者的GO条目集中在磷酸酶结合、蛋白丝氨酸/苏氨酸激酶活性、泛素蛋白连接酶结合等生物学过程;KEGG富集通路集中在PI3K-Akt信号通路、MAPK信号通路、流体剪切应力和动脉粥样硬化信号通路、Th17细胞分化等信号通路。结论本文初步阐释了人参、红参及黑参抗肺气虚的作用机制及物质基础。并且根据活性成分及作用机制推测治疗作用黑参>红参>人参。三者可能通过机体免疫应答、炎症�
Objective To explore the mechanism and material basis of ginseng,red ginseng and black ginseng against Lung Qi deficiency by network pharmacology.Methods ①The chemical constituents and targets of ginseng,red ginseng and black ginseng were screened by TCMSP and related literatures.②The human Gene database Gene Cards was used to obtain the related targets of chronic bronchitis,and then the intersection of disease targets with ginseng,red ginseng and black ginseng was obtained.③After obtaining the common target of each drug and disease,the protein interaction was analyzed in STRING database,and the drug-component-disease-target interaction network was established by using Cytoscape software to obtain the main effective components for treating diseases.④GO and KEGG enrichment analysis was performed by R language analysis technology.Results The active components of each drug were screened by TCMSP and related literatures,and then sorted according to the Degree value of drug-component-disease-target interaction network.The top ten ingredients in ginseng were ginsenoside Rh4,Rg5,kaempferol,ginsenoside Rh1,Ra2,notoginseng R2,notoginseng R6,Ra0,Ral,Ra3.The first ten components in red ginseng were ginsenoside Rh4,Rh1,notoginseng R2,ginsenoside Rg2,Rb1,Rb2,Rc,Re,Rs1,beta-sitosterol.The first ten components of black ginseng were ginsenoside Rh3,Rh4,Rk3,Rk1,Rg5,F4,20(S)-Rh2,20(R)-Rh2,Rh1,Rg3.The potential targets of ginseng,red ginseng and black ginseng in treating chronic bronchitis were 46,39 and 53,respectively.According to Degree>15,In addition to the common target proteins MAPK1,MAPK8,STAT3,VEGFA,MTOR and MMP9,CASP3 of panax ginseng,CASP8 and BCL2 L1 of red ginseng,AKT1,MMP2 and MAPK14 of black ginseng were screened.Through GO function and KEGG pathway enrichment analysis,the top 20 GO items and the top 10 KEGG enrichment pathways of ginseng,red ginseng and black ginseng were screened according to P values.The GO items of the three materials focus on biological processes such as phosphatase binding,protein serine
作者
陈宏雨
刘琳琳
窦德强
CHEN Hong-yu;LIU Lin-lin;DOU De-qiang(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)
出处
《人参研究》
2022年第1期2-10,共9页
Ginseng Research
基金
国家自然科学基金,项目编号:81773858、82073985
辽宁省重点研发计划项目,项目编号:2020JH2/10300055。
关键词
网络药理学
人参
红参
黑参
肺气虚
慢性支气管炎
network pharmacology
Ginseng
Red ginseng
Black ginseng
Lung qi deficiency
Chronic bronchitis
