摘要
目的观察酒精中毒后大鼠脑血管及细胞相关超微结构改变及其机制。方法Wistar雄性大鼠酒精灌胃4 w造成慢性酒精中毒模型。通过透射电子显微镜观察酒精中毒后大鼠脑血管及细胞损伤相关超微结构改变。结果酒精中毒组大鼠小脑毛细血管内皮细胞中细胞器明显减少。细胞核形状不规则,核仁不明显,形状不规则。额叶内的线粒体明显肿胀,两层单位膜不清晰,嵴断裂,数目减少,嵴上附着的核糖体脱落。管状粗面内质网断裂,长度减短,彼此间隔增宽,数量明显减少,管膜外表面附着的核糖体脱落。海马内可见轻度脱髓鞘改变,多层质膜构成的板层样髓鞘有轻度的脱落及破坏。结论酒精中毒后可引起脑血管及细胞相关超微结构改变、损伤。为后续预防及治疗提供基础研究理论及思路。
Objective To investigate the ultrastructural changes of brain vessels and cells after alcoholism in rats and the underlying mechanisms.Methods Wistar male rats were given alcohol intragastrically for 4 weeks to induce chronic alcoholism model.To observe the ultrastructural changes related to brain vascular and cells damage after alcohol intoxication in rats by a transmission electron microscope.Results The shape of cerebellar capillary endothelial cells was irregular in the alcohol intoxication group,there were fewer organelles in the cytoplasm.The nuclei are irregularly shaped,the nuclei are not obvious,irregular shape.The mitochondria in the frontal lobes are swollen,crest fracture,reduced number,the ribosomes attached to the crest fall off.The tubular rough endoplasmic reticulum is fractured,the interval between each other is widened,the quantity is reduced,the ribosomes attached to the outer surface of the tube membrane fall off.Mild demyelinating changes were observed in the hippocampus,and the lamellar myelin sheath composed of multiple plasma membranes was slightly detached and damaged.Conclusion Alcoholism can cause the change and damage of the ultrastructure of cerebrovascular and related cells.To provide basic research theories and ideas for subsequent prevention and treatment.
作者
赵丽
关继奎
陈嘉峰
ZHAO Li;GUAN Jikui;CHEN Jiafeng(The First People’s Hospital of Wuhu,Wuhu 241000,China)
出处
《中风与神经疾病杂志》
CAS
2021年第10期1110-1112,共3页
Journal of Apoplexy and Nervous Diseases
关键词
酒精中毒
脑血管及细胞损伤
超微结构
Alcoholism
Cerebrovascular and cellular damage
Ultramicrostructure
