摘要
探究榛蘑多糖对乙醇所致大鼠血管内皮细胞损伤的保护作用。将40只SD大鼠随机分成4组(正常对照组、损伤组、榛蘑多糖低剂量组、榛蘑多糖高剂量组)。除正常对照组外的其余各组均按10 mL/kg mb灌胃体积分数40%乙醇诱导血管内皮细胞损伤。榛蘑多糖低、高剂量组分别以100、400 mg/kg mb灌胃榛蘑多糖,其余组以等体积生理盐水代替,实验共进行4周。使用苏木精-伊红(hematoxylin-eosin,HE)染色观察颈动脉组织病理变化;检测血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,T-CHO)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、内皮型一氧化氮合酶、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、一氧化氮(NO)、内皮素1(endothelin 1,ET-1)、超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)水平。此外,以600μmol/mL乙醇诱导人脐静脉内皮细胞损伤模型,分析不同剂量(100、400μg/mL)榛蘑多糖对细胞活性氧(reactive oxygen species,ROS)、线粒体跨膜电位、细胞凋亡以及凋亡相关蛋白B淋巴细胞瘤2(B cell lymphoma 2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)和Caspase-3表达的影响。结果表明:榛蘑多糖减轻乙醇所致的血管内膜损伤,降低T-CHO、TG、LDL-C、iNOS、NO、ET-1和MDA水平,提高HDL-C和SOD活性;在体外条件下,榛蘑多糖降低细胞ROS水平,抑制乙醇所致的线粒体跨膜电位的下降和细胞凋亡,并提高Bcl-2/Bax,下调Cleaved caspase-3表达水平。综上,榛蘑多糖对乙醇诱导的大鼠血管内膜损伤有保护作用,机制可能与其降脂、抗氧化和抗凋亡作用有关。
The aim of this study is to evaluate the protective effect of Armillaria mellea polysaccharides(AMP)against ethanol-induced vascular endothelial cell injury in rats.Altogether 40 SD rats were randomly divided into 4 groups:normal control,injury,low-and high-dose AMP.The rats from all groups except the normal control group were gavaged with 40%ethanol at 10 mL/kg mb to induce endothelial cell injury.The rats in the low-and high-dose groups were gavaged with AMP at 100 and 400 mg/kg mb,respectively,while the remaining groups were given an equal volume of physiological saline.The administration period lasted for 4 weeks.The histopathological changes of carotid arteries were observed by hematoxylin-eosin(HE)staining.Serum triglycerides(TG),total cholesterol(T-CHO),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),endothelial nitric oxide synthase(eNOS),inducible nitric oxide synthase(iNOS),nitric oxide(NO),endothelin 1(ET-1),superoxide dismutase(SOD),and malondialdehyde(MDA)levels were detected.In addition,the effects of different doses(100 and 400μg/mL)of AMP on the levels of reactive oxygen species(ROS),mitochondrial transmembrane potential,apoptosis,and the expression of apoptosis-associated proteins such as B cell lymphoma 2(Bcl-2),Bcl-2 associated X protein(Bax),and caspase-3 in human umbilical vein endothelial cell injured by 600μmol/mL ethanol were investigated.Results:AMP attenuated ethanol-induced endothelial cell injury,decreased T-CHO,TG,LDL-C,iNOS,NO,ET-1,and MDA levels,and increased HDL-C and SOD activity.In vitro,AMP reduced cellular ROS levels,inhibited the ethanol-induced decrease in mitochondrial transmembrane potential and apoptosis,increased Bcl-2/Bax ratio and down-regulated the expression of cleaved caspase-3.Conclusion:AMP have a protective effect against ethanol-induced endothelial cell injury in rats,and the mechanism may be related to their lipid-lowering,antioxidant,and anti-apoptotic effects.
作者
张俊慧
陈然然
丛贺
沈明花
ZHANG Junhui;CHEN Ranran;CONG He;SHEN Minghua(Medical College,Yanbian University,Yanji 133002,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第7期127-134,共8页
Food Science
基金
国家自然科学基金地区科学基金项目(81760031)
吉林省中医药管理局中医药科技项目(2022094)。
关键词
榛蘑多糖
血管内皮细胞损伤
乙醇
降脂
抗氧化
Armillaria mellea polysaccharides
vascular endothelium cell injury
ethanol
lipid-lowering
antioxidant
