摘要
目的明确妊娠期实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis, EAE)小鼠的神经功能的变化情况,并进一步探索其可能的作用机制。方法采用MOG35-55多肽诱导制作雌性EAE小鼠模型,按雌雄小鼠2:1比例合笼受孕,以成功妊娠的小鼠为妊娠组(n=10);未配孕的小鼠为对照组(n=10)。比较2组神经功能评分(5分评分法),脊髓实质内炎症细胞的浸润(HE法)及神经脱髓鞘(Bielshowsky’s-Luxol fast blue staining染色)情况,血清IFN-γ、IL-17、IL-10浓度(ELISA法),脾脏中Treg细胞数百分比(流式细胞术)。结果妊娠组小鼠神经功能缺损程度在妊娠后第10、13天较对照组显著降低(P<0.05),且妊娠组小鼠脊髓病灶区炎症细胞浸润程度较对照组显著降低(P<0.05);2组小鼠的脑和脊髓白质内均可见神经脱髓鞘改变,妊娠组的脱髓鞘程度较对照组轻(P<0.05);妊娠组小鼠血清的IFN-γ、IL-17浓度较对照组显著降低(P<0.05),但血清IL-10浓度较对照组显著升高(P<0.05);妊娠组小鼠脾脏中调节性T细胞(Treg细胞)数百分比较对照组显著升高(P<0.05)。结论妊娠期EAE小鼠的临床症状缓解且脊髓实质内炎症反应减轻;其可能的作用机制为上调Treg细胞的抗炎因子和抑制促炎因子的分泌。
Objective To clarify the changes in neurological function in pregnant mice with experimental autoimmune encephalomyelitis(EAE),and to further explore its possible mechanism of action.Methods EAE was induced by MOG35-55 polypeptide in female mice.Then, the male and female mice were mated at a ratio of 2:1.The pregnant mice were selected as the pregnancy group(n=10),and the non-pregnant mice as the control group(n=10).Neurological function was determined using a five-point scale.Demyelination was observed by Bielshowsky’s-Luxol fast blue staining.Serum concentrations of IFN-γ,IL-17 and IL-10 were measured by ELISA.The percentage of Treg cells in spleen was detected by flow cytometry.Results Compared with the control group, the degree of neurological deficit on the 10 th and 13 th days of gestation and the infiltration of inflammatory cells in spinal cord lesions obviously decreased in the pregnancy group(P<0.05).Nerve demyelination was observed in the white matter of brain and spinal cord in both groups.However, the degree of demyelination in the pregnancy group was less than that in the control group(P<0.05).Serum concentrations of IFN-γ and IL-17 in the pregnancy group were lower than those in the control group, but IL-10 level in the pregnancy group was higher than that in the control group(P<0.05).Furthermore, the percentage of Treg cells in the spleen in the pregnancy group was higher than that in the control group(P<0.05).Conclusion The clinical symptoms and inflammatory reaction in spinal cord parenchyma are relieved in pregnant EAE mice by up-regulating Treg cells-produced anti-inflammatory factors and inhibiting the secretion of pro-inflammatory factors.
作者
张洪连
周鑫华
谢琛
江建香
梅竹君
刘诗英
胡凡
曹文锋
ZHANG Hong-lian;ZHOU Xin-hua;XIE Chen;JIANG Jian-xiang;MEI Zhu-jun;LIU Shi-ying;HU Fan;CAO Wen-feng(Department of Neurology,Jiangxi People’s Hospital,Affiliated People’s Hospital Nanchang University,Nanchang 330006,China)
出处
《南昌大学学报（医学版）》
2021年第6期19-23,31,共6页
Journal of Nanchang University：Medical Sciences
基金
江西省卫生计生委科技计划(20191003)。
