摘要
目的探讨外周血LncRNA-p3134表达水平在妊娠期糖尿病(GDM)诊断中的价值。方法选取GDM孕妇82例为GDM组,非GDM孕妇116例为对照组。检测2组外周血LncRNA-p3134的表达水平;分析LncRNA-p3134表达与胰岛素抵抗指数(HOMA-IR)、胰岛素敏感性指数(ISOGTT)和胰岛β细胞功能指数(HOMA-β)水平、GDM发生的相关性;分析LncRNA-p3134表达对GDM发生的影响以及LncRNA-p3134在GDM诊断中的作用。结果GDM组LncRNA-p3134相对表达水平高于对照组,差异有统计学意义(P<0.05)。LncRNA-p3134表达与ISOGTT、HOMA-β呈负相关(P<0.05),与HOMA-IR、GDM发生呈正相关(P<0.05)。校正混杂因素后,LncRNA-p3134表达是GDM发生的独立影响因素(P<0.05),LncRNA-p3134表达相对量越高则GDM发生风险越大。受试者工作特征(ROC)曲线分析显示,LncRNA-p3134诊断GDM的曲线下面积(AUC)为0.853,敏感度和特异度分别为75.0%、83.0%。结论LncRNA-p3134在GDM中表达水平较非GDM高,可能通过影响胰岛素敏感性以及胰岛β细胞功能导致GDM的发生,其在临床上可能可作为GDM诊断的候选标志物。
Objective To explore the value of peripheral blood lncRNA-p3134 in diagnosing gestational diabetes mellitus(GDM).Methods A total of 82 pregnant women with GDM were selected as GDM group,and 116 non-GDM pregnant women were selected as control group.The expression levels of lncRNA-p3134 in peripheral blood of the two groups were detected;the correlations between the expression of lncRNA-p3134 and insulin resistance index(HOMA-IR),insulin sensitivity index(ISOGTT),isletβcell function index(HOMA-β)and the occurrence of GDM were analyzed.The effect of lncRNA-p3134 expression on the occurrence of GDM and the role of lncRNA-p3134 in the diagnosis of GDM were analyzed.Results The relative expression level of lncRNA-p3134 in the GDM group was significantly higher than that in the control group(P<0.05).LncRNA-p3134 expression was negatively correlated with ISOGTT and HOMA-β(P<0.05),and positively correlated with HOMA-IR and GDM(P<0.05).After adjusting confounding factors,lncRNA-p3134 expression was an independent factor affecting the occurrence of GDM(P<0.05),and the higher the relative expression level of lncRNA-p3134 was the higher the risk of GDM would be.Receiver operating characteristic(ROC)curve analysis showed that the area under the curve(AUC)of lncRNA-p3134 for GDM diagnosis was 0.853,sensitivity and specificity were 75.0%and 83.0%,respectively.Conclusion The expression level of lncRNA-p3134 in GDM is higher than that of non-GDM,which may lead to the occurrence of GDM by affecting insulin sensitivity and isletβcell function,and it may be used as a candidate marker for the diagnosis of GDM in clinical practice.
作者
谢芳
李强
赵爱国
杨卫华
XIE Fang;LI Qiang;ZHAO Aiguo;YANG Weihua(Department of Endocrinology and Metabolism,Shenzhen Hospital of University of Chinese Academy of Sciences,Shenzhen,Guangdong,518107)
出处
《实用临床医药杂志》
CAS
2021年第20期106-110,共5页
Journal of Clinical Medicine in Practice
