摘要
目的检测卵巢癌中长链非编码RNA(lncRNA)肺癌转移相关转录本1(MALAT1)和microRNA-200c(miR-200c)的表达水平,并探究其临床意义。方法经手术切除的上皮性卵巢癌组织85例、卵巢良性肿瘤组织60例和正常卵巢组织60例。实时定量PCR(qRT-PCR)技术检测3组卵巢组织lncRNA MALAT1、miR-200c水平;分析卵巢癌组织lncRNA MALAT1、miR-200c表达与患者临床病理特征及预后的关系。结果卵巢癌组织中lncRNA MALAT1、miR-200c表达水平均显著高于卵巢良性肿瘤组织和正常卵巢组织(P<0.05);卵巢良性肿瘤组织中lncRNA MALAT1、miR-200c表达水平均显著高于正常卵巢组织(P<0.05)。卵巢癌患者卵巢组织中lncRNA MALAT1和miR-200c表达水平无线性关系(P>0.05)。卵巢癌组织lncRNA MALAT1、miR-200c表达水平与卵巢癌患者的肿瘤大小、病理分期、分化程度、淋巴结转移情况均有关(P<0.05),与患者的年龄、有无腹水、组织学类型无关(P>0.05)。lncRNA MALAT1低表达患者的预后显著优于lncRNA MALAT1高表达患者(P<0.05)。miR-200c高表达患者的预后显著优于miR-200c低表达患者(P<0.05)。结论 lncRNA MALAT1、miR-200c表达异常与上皮性卵巢癌的发生、发展有关,可能成为上皮性卵巢癌临床治疗及预后的新靶点。
Objective To investgate the expression levels of metastasis-associated lung adenocarcinoma transcript 1(MALAT1)and microRNA-200 c(miR-200 c)of long non-coding RNA(lncRNA)in ovarian cancer,and explore its clinical significance.Methods A total of 85 cases of epithelial ovarian cancer tissues,60 cases of benign ovarian tumor tissues and 60 cases of normal ovarian tissues were enrolled in the study.The expression levels of lncRNA MALAT1 and miR-200 c were detected by real time quantitative PCR(qRT-PCR)technique,and the correlation between lncRNA MALAT1,miR-200 c expressions and clinicopathological characteristics as well as prognosis of patients with ovarian cancer was analyzed.Results The expression levels of lncRNA MALAT1 and miR-200 c in ovarian cancer tissues were significantly higher than those in benign ovarian tumor tissues(P<0.05)and normal ovarian tissues(P<0.05),and the expression levels of lncRNA MALAT1 and miR-200 c in benign ovarian tumor tissues were significantly higher than those in normal ovarian tissues(P<0.05).Theere was no linear relationship between the expression levels of lncRNA MALAT1 and those of miR-200 c in ovarian cancer tissues(P>0.05).The expression levels of lncRNA MALAT1 and miR-200 c in ovarian cancer tissues was related to the tumor size,pathology staging,differentiation degree and lymph node metastasis,however,which were not related to patient’s age,the presence of ascites,and the histologic type of tumor(P>0.05).The prognosis of patients with low expression of LncRNA MALAT1 was superior to that of patients with high expression of LncRNA MALAT1(P<0.05).Moreover the prognosis of patients with high expression of miR-200 c was superior to that of patients with low expression of miR-200 c(P<0.05).Conclusion The abnormal expression of MALAT1 and miR-200 c in LncRNA is related to the pathogenesis and development of epithelial ovarian cancer,it may become an new target for clinical treatment and prognosis improvement in patients with epithelial ovarian cancer.
作者
张宝玺
刁海丹
李江宁
ZHANG Baoxi;DIAO Haidan;LI Jiangning(Department of Gynaecology,The Third People’s Hospital of Dalian City,Liaoning,Dalian116031,China)
出处
《河北医药》
CAS
2019年第4期489-493,共5页
Hebei Medical Journal
