摘要
试验研究发酵麸皮多糖(FWBP)对大鼠肾脏抗氧化功能的影响。选取48只健康雄性Wistar断奶大鼠,按照体重相近原则,随机分为6组,分别为空白对照组(生理盐水)、FWBP组(分别为10、20、40和80 mg/kg BW)以及维生素C(VC)组(100 mg/kg BW),每组8个重复,每个重复1只。试验期21 d。结果显示,与空白对照组相比,灌胃10、20、40 mg/kg BW FWBP组大鼠肾脏中的谷胱甘肽(GSH)含量显著提高(P<0.05);灌胃40 mg/kg BW FWBP的大鼠肾脏中8-OHdG含量显著降低(P<0.05)。与空白对照组相比,灌喂20 mg/kg BW FWBP大鼠肾脏中谷氨酸-半胱氨酸连接酶催化亚基(GCLC)和醌氧化还原酶1(NQO1)的mRNA表达量均显著提高(P<0.05)。研究表明,FWBP可以通过激活核因子E2相关因子2(Nrf2),促进GCLC、谷氨酸-半胱氨酸连接酶调节亚基(GCLM)和NQO1的转录和表达,诱导GSH的分泌,减轻DNA损伤,进而提高大鼠肾脏抗氧化能力,有效保护肾脏的健康。
The experiment was to study the effect of fermented bran polysaccharide(FWBP)on antioxidant function of rat kidney.48 healthy male Wistar weaned rats were randomly divided into six groups according to the principle of similar weight,namely blank control group(normal saline),FWBP group(10,20,40 and 80 mg/kg BW respectively)and vitamin C(VC)group(100 mg/kg),with eight replicates in each group and one rat in each replicate.The feeding period was 21 d.The results showed that compared with blank control group,the glutathione(GSH)content of 10,20 and 40 mg/kg BW FWBP was significantly increased by intragastric administration(P<0.05).The content of 8-OHDG in kidney of 40 mg/kg BW FWBP was significantly decreased(P<0.05).Compared with blank control group,mRNA expression levels of glutamate-cysteine ligase catalyzed subunit(GCLC)and quinone oxidoreductase 1(NQO1)in kidney of FWBP rats fed 20 mg/kg BW were significantly increased(P<0.05).The experiment indicates that FWBP can promote the transcription and expression of GCLC,glutamate-cysteine ligase regulatory subunit(GCLM)and NQO1 by activating e2-related factor 2(Nrf2),induce the secretion of GSH,and reduce DNA damage,thus improving the antioxidant capacity of rat kidney and effectively protecting kidney health.
作者
张佳
段元霄
王文文
王园
安晓萍
齐景伟
ZHANG Jia;DUAN Yuan-xiao;WANG Wen-wen;WANG Yuan;AN Xiao-ping;QI Jing-wei
出处
《饲料研究》
CAS
北大核心
2021年第19期71-74,共4页
Feed Research
基金
内蒙古自然科学基金项目“小麦麸皮阿魏酸低聚糖通过调控肠道屏障功能缓解羔羊大肠杆菌性腹泻(项目编号:2020MS03041)”
内蒙古自治区关键技术攻关计划项目“基于AI技术的肉羊高产关键技术开发与示范(项目编号:2020GG0030)”
内蒙古自治区科技重大专项“草食家畜智慧养殖关键技术开发及示范(项目编号:2020ZD0004)”。
关键词
发酵麸皮多糖
大鼠
肾脏
抗氧化
fermented bran polysaccharide
rats
kidney
antioxidation
