摘要
本文以紫球藻FJ-12为藻株,在发光二极管(LED)绿光条件下,研究不同氮浓度(0、0.5、1和1.5 g/L)对紫球藻细胞的生长和生物活性产物(藻胆蛋白、胞外硫酸酯多糖和多不饱和脂肪酸)合成的影响。结果表明:在氮浓度为1 g/L的条件下,紫球藻可获得最大生物量,为3.09 g/L。藻细胞在氮浓度为1 g/L和1.5 g/L的条件下培养可合成最高的藻蓝蛋白、藻红蛋白、别藻蓝蛋白、胞外硫酸酯多糖和油脂浓度与产量。紫球藻在1.5 g/L氮浓度下可合成最大的花生四烯酸(ARA)和二十碳五烯酸(EPA)占比,但ARA和EPA产量分别与1 g/L氮浓度下的ARA和EPA产量无显著差异。综上可得,在LED绿光下,1 g/L KNO_(3)是紫球藻最适合生长和活性物质合成的氮浓度,可实现紫球藻多联产高效协同合成藻胆蛋白、胞外硫酸酯多糖和多不饱和脂肪酸,解决紫球藻协同合成活性物质的瓶颈问题,绿光和氮浓度为规模化养殖紫球藻和开发其活性物质提供实践证据。
Using Porphyridium cruentum as the algae strain,the aim of this work was to investigate the effects of different nitrogen concentrations(0,0.5,1 and 1.5 g/L of KNO_(3))on the growth and bioactive compounds(phycobiliproteins,extracellular sulfate polysaccharides and polyunsaturated fatty acids)biosynthesis of P.cruentum under the light-emitting diodes(LED)green light.The results showed that P.cruentum could obtain the maximum biomass(3.09 g/L)in the nitrogen concentration of 1 g/L under the LED green light.Moreover,microalga cultivated under LED greed light at 1 g/L and 1.5 g/L KNO_(3)achieved the highest concentrations and productivity of phycocyanin,phycoerythrin,allophycocyanin,extracellular sulfate polysaccharide and lipid.Furthermore,P.cruentum attained the highest contents of ARA and EPA at 1.5 g/L KNO_(3).However,no significant difference in ARA content was found by microalga between 1 g/L KNO_(3)and 1.5 g/L KNO_(3).Based on these results,it could draw a conclusion that P.cruentum grown in 1 g/L KNO_(3)under LED green light exhibited the excellent growth characteristic and ability to maximally produce phycobiliproteins,extracellular sulfate polysaccharides and polyunsaturated fatty acids.This work addressed the bottleneck about the coproduction of P.cruentum-derived bioactive compounds,offering the scientific reference to cultivate P.cruentum and exploit its bioactive substances for food,feed and cosmetic in future.
作者
郭帅
黄子诚
谢点
吴黎明
郑明敏
陈必链
何勇锦
GUO Shuai;HUANG Zi-cheng;XIE Dian;WU Li-ming;ZHENG Ming-min;CHEN Bi-lian;HE Yong-jin(College of Life Sciences,Fujian Normal University;Engineering Research Center of Industrial Microbiology of Ministry of Education,Fujian Normal University,Fuzhou 350117,China)
出处
《天然产物研究与开发》
CAS
CSCD
2021年第10期1751-1757,共7页
Natural Product Research and Development
基金
福建省科学计划引导性项目(2020N0008)
国家自然科学基金(31900092)
2021年大学生创新创业训练计划(cxxl-2021477)。
关键词
紫球藻
氮浓度
培养
生物活性产物
Porphyridium cruentum
nitrogen concentrations
cultivation
bioactive substances
