摘要
巴夫杜氏藻(Dunaliella parva)是一类独特的耐盐绿藻,富含多种生物活性物质,具有宝贵的工业应用价值。随着转录组测序技术的广泛应用,越来越多的藻类功能基因被发掘。然而,巴夫杜氏藻在不同生长时期的转录组测序仍见未报道,这极大限制了该藻株生长发育机制的深入研究。该研究综合运用形态学、组学及生物信息学技术,对生长初期、中期和末期的巴夫杜氏藻进行转录组测序分析。结果显示,9个cDNA测序文库共获得90153条UniGenes,其中coding domain sequences 49643条,66.36%可被七大生物数据库注释,de novo组装了272条Contigs,其中N_(50)为1305 bp,GC百分比52.32%。基于基因的差异表达值可将UniGene划分为4簇,以生长初期实验组为对照,筛选获得下调基因数(52891条)高于上调基因数(33496条),共有基因数20759条。生长中期与初期及生长末期与初期间的基因本体论富集程度最高的均是细胞膜的整体成分,生长中期与初期及生长末期与初期间的KEGG(Kyoto Encyclopedia of Genes and Genomes)通路富集程度最高的分别是剪接体和RNA转运。该研究获得的巴夫杜氏藻转录组测序数据及组装质量较高,筛选的差异表达基因及预测的功能信息可为该藻生长发育调控机制的研究、资源品质的分子改良及工业应用奠定基础。
As a class of unique salt-tolerance green alga,Dunaliella parva has industrial production potential due to its rich bioactive substances.With the wide application of transcriptomic sequencing,more and more algae functional genes have been discovered.However,the transcriptomic sequencing of D.parva during different growth phases has not been reported yet,which greatly limits the further research on its growth and development mechanism.In this study,transcriptomic sequencing of the algal samples at the early,mid and late growth stages based on morphological,omics and bioinformatics methods was performed.A total of 90153 unigenes with 49643 coding domain sequences from 9 cDNA libraries were obtained in this alga.66.36%of them could be annotated by the seven major bio-databases.272 contigs were de novo assembled with a 1305 bp N_(50)and a GC percentage of 52.32%.The unigenes could be divided into 4 clusters based on their fragments per kilobase of transcript per million fragments values.Compared with the early-stage group,the number of down-regulated genes(52891)was larger than that of the up-regulated ones(20759).Integral component of membrane was the most significant ontology in both the mid and late stage sample via gene ontology enrichment analyzing compared with the early-stage sample.Additionally,splicesome and RNA transport were the most significant pathways in the mid and late stage sample,respectively,via KEGG pathway enrichment analysis.The quality of the transcriptomic sequencing data and de novo assembly was high.The screened differentially expressed genes and their predicted functional information will lay a foundation for the further research on growth and development mechanism analysis,algal strain molecular improvement and industrial application of this alga.
作者
宋韡
汤丽群
高帆
SONG Wei;TANG Liqun;GAO Fan(Shanxi Sports Vocational School,Taiyuan 030006,China;School of Life Science,Shanxi University,Taiyuan 030006,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2022年第4期82-89,共8页
Food and Fermentation Industries
基金
国家自然科学基金项目(31800657)
2019年山西省高等学校科技创新项目(2019L0041)
山西省科技攻关计划项目(201903D321069)。
关键词
巴夫杜氏藻
转录组分析
差异表达基因
生长时期
Dunaliella parva
transcriptomic analysis
differentially expressed genes
growth period
