摘要
研究网地藻多糖对α-葡萄糖苷酶活性的抑制作用。以网地藻为原料,采用超声辅助水提、醇沉、脱色、脱蛋白、干燥后制得网地藻多糖。利用对-硝基苯酚-α-D-葡萄糖苷(p-nitrophenyl-α-D-glucopyranoside,PNPG)为底物建立酶-抑制剂体外模型,采用双倒数作图法确定其抑制类型。结果表明:网地藻多糖抑制α-葡萄糖苷酶活性的大小会受到反应pH值、反应温度、反应时间及网地藻多糖质量浓度的影响,在反应pH值为6.8、反应温度为37℃、反应时间为20 min的条件下网地藻多糖对α-葡萄糖苷酶具有一定的抑制作用,其IC50为22.64 mg/mL。抑制动力学试验表明网地藻多糖对α-葡萄糖苷酶的抑制作用为混合型抑制。结果表明网地藻多糖具有一定的降糖作用。
The inhibitive effects in vitro of Dictyota dichotoma polysaccharides onα-glucosidase activity was studied.The polysaccharide from Dictyota dichotoma was extracted by ultrasonic-assisted technology,ethanol precipitation,deproteinization,decolorization and desiccation.Using p-nitrophenyl-α-D-glucopyranoside(PNPG)as the substrate,the enzyme-inhibitor model was used to study the inhibition ability of polysaccharide from Dictyota dichotoma of theα-glucosidase and the double reciprocal plot method was used to determine the type of inhibition.The inhibitory effect of Dictyota dichotoma polysaccharides was impacted by pH,temperature,time and polysaccharide concentration.The optimal pH,temperature,reaction time and concentration for Dictyota dichotoma polysaccharides inhibitory effect onα-glucosidase were pH 6.8,37℃,20 min,respectively.The half-maximal inhibitory concentration(IC50)value of Dictyota dichotoma polysaccharide was 22.64 mg/mL,showing a good hypoglycemic function.The inhibition type was mixed inhibition.The enzyme-inhibitor model showed that the polysaccharide from the Dictyota dichotoma had a certain inhibition effect on the activity ofα-glucosidase.
作者
冯学珍
覃慧逢
冯书珍
FENG Xue-zhen;QIN Hui-feng;FENG Shu-zhen(Guangxi University of Science and Technology,Liuzhou 545006,Guangxi,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第3期35-40,共6页
Food Research and Development
基金
广西植物功能物质研究与利用重点实验室开放基金课题（FPRU2016-2）
广西高校中青年教师基础能力提升项目（2016LX168）
广西科技大学博士基金项目（校科博17Z05）
关键词
Α-葡萄糖苷酶
抑制类型
网地藻
多糖
抑制常数
α-glucosidase
inhibition type
Dictyota dichotoma
polysaccharide
inhibition constant
