摘要
目的探究系统性红斑狼疮(SLE)患者血清微小RNA-23b(miR-23b)、趋化因子配体19(CCL19)水平与疾病活动度、补体C3、补体C4的相关性。方法选取2019年1月至2021年1月在本院风湿免疫科初诊、尚未治疗的SLE患者176例为SLE组,同期体检健康者124例为健康对照组。比较两组血清miR-23b、CCL19、补体C3、补体C4水平;采用SLE疾病活动指数2000(SLEDAI-2000)评分评估SLE患者的疾病活动度,比较不同活动度组血清指标水平;并分析miR-23b、CCL19与疾病活动度、补体C3、补体C4的相关性以及血清指标对SLEDAI-2000评分的影响。结果SLE组血清miR-23b、补体C3、补体C4水平低于对照组,血清CCL19水平高于对照组,差异有统计学意义(P<0.05)。血清miR-23b、补体C3、补体C4水平在重度活动组<中度活动组<轻度活动组<无活动组,血清CCL19水平在重度活动组>中度活动组>轻度活动组>无活动组,差异有统计学意义(P<0.05)。Pearson相关性分析显示,miR-23b与补体C3、补体C4呈正相关(r=0.616、0.712,P<0.05),CCL19与补体C3、补体C4呈负相关(r=-0.342、-0.516,P<0.05)。Spearman等级相关分析显示,miR-23b与疾病活动度呈负相关(r=-0.785,P<0.05),CCL19与疾病活动度呈正相关(r=0.740,P<0.05)。多元线性回归分析显示,miR-23b、CCL19、补体C3、补体C4是SLEDAI-2000评分的影响因素(P<0.05);miR-23b、补体C3、补体C4负向影响SLEDAI-2000评分(B=-5.750、-2.854、-19.490,95%CI=-8.075~-3.425、-5.217~-0.492、-25.431~-13.550,均P<0.05),CCL19正向影响SLEDAI-2000评分(B=0.030,95%CI=0.025~0.036,P<0.05)。结论SLE患者血清miR-23b水平降低、CCL19水平升高,miR-23b、CCL19与疾病活动度、补体C3、补体C4存在相关性,且miR-23b、CCL19、补体C3、补体C4共同影响疾病活动度。
Objective To explored the correlation of serum microRNA-23b(miR-23b),C-C chemokine ligand 19(CCL19)levels and disease activity,complement C3,and complement C4 in patients with systemic lupus erythematosus(SLE).Methods 176 patients with untreated SLE who were newly diagnosed in the Department of Rheumatology and Immunology of our hospital from January,2019 to January,2021 were selected as the SLE group,while 124 healthy patients of physical examination during the same period were selected as the healthy control group.The serum levels of miR-23b,CCL19,complement C3,and complement C4 between the two groups were compared;The SLE disease activity indeX_(2)000(SLEDAI-2000)score was used to assess the disease activity in SLE patients and the serum index levels among different activity groups were compared;The correlation between miR-23b,CCL19 and disease activity,complement C3,complement C4,and the influence of serum indicators on the SLEDAI-2000 score were analyzed.Results The levels of serum miR-23b,complement C3,and complement C4 in the SLE group were lower than those of the control group,while serum CCL19 level was higher than that of the control group(P<0.05).The levels of serum miR-23b,complement C3,and complement C4 were following the order as:the heavy activity group<moderate activity group<light activity group<no activity group.The levels of serum CCL19 was following the order as:the heavy activity group>moderate activity group>light activity group>no activity Group,and the difference was statistically significant(P<0.05).Pearson correlation analysis showed that miR-23b was positively correlated with complement C3 and complement C4(r=0.616,0.712,P<0.05),while CCL19 was negatively correlated with complement C3 and complement C4(r=-0.342,-0.516,P<0.05).Spearman rank correlation analysis showed that miR-23b was negatively correlated with disease activity(r=-0.785,P<0.05),while CCL19 was positively correlated with disease activity(r=0.740,P<0.05).Multiple linear regression analysis showed that miR-23b,CCL19,complem
作者
矫淑媛
郝铁
JIAO Shuyuan;HAO Tie(Medical Laboratory,Anshan Central Hospital,Anshan 114001,China)
出处
《标记免疫分析与临床》
CAS
2021年第9期1549-1555,1594,共8页
Labeled Immunoassays and Clinical Medicine
