摘要
【目的】探讨黄芪组分对糖尿病肾病大鼠循环及肾脏局部肾素-血管紧张素系统的影响。【方法】将55只SD大鼠随机分为正常组(10只)与造模组(45只)。将造模组大鼠采用链脲佐菌素腹腔注射法构建成糖尿病肾病模型。成功造模后,将造模组大鼠随机分为模型组、黄芪多糖组、黄芪总皂苷组。2个治疗组对应给予相应药物灌胃,正常组和模型组给予等体积的蒸馏水灌胃。连续给药12周。治疗期间,每4周动态观察尿量及24 h尿微量白蛋白,12周后计算肾质量/体质量比值,采用放射免疫法检测血浆及肾组织中肾素、血管紧张素Ⅱ(AngⅡ)含量,分别采用实时定量聚合酶链反应(RT-PCR)法与免疫组织化学法检测肾组织中血管紧张素转化酶(ACE)mRNA与蛋白表达水平。【结果】与正常组比较,模型组大鼠尿量、24 h尿微量白蛋白量、肾质量/体质量比值增加(P<0.01),肾组织病理可见轻度系膜增生,细胞外基质略增多,血浆及肾组织中肾素、AngⅡ含量,肾组织中ACE mRNA与蛋白表达水平均明显增高(P<0.01)。与模型组比较,黄芪多糖组、黄芪总皂苷组大鼠尿量、24 h尿微量白蛋白量、肾质量/体质量比值降低(P<0.01),肾脏病理形态学得到一定改善,血浆及肾组织中AngⅡ含量、肾组织中ACE mRNA与蛋白表达水平均降低(P<0.01)。黄芪总皂苷组大鼠血浆及肾组织肾素活性较模型组显著降低(P<0.01),而黄芪多糖组血浆及肾组织肾素活性与模型组比较,差异无统计学意义(P> 0.05)。【结论】黄芪多糖和黄芪总皂苷可防止或延缓糖尿病肾病的进展,其降低糖尿病肾病微量白蛋白尿的机制可能与抑制肾素-血管紧张素系统的过度激活有关。
Objective To explore the effects of Radix Astragali constituents on circulatory and local renal reninangiotensin system(RAS) in rats with diabetic nephropathy. Methods Fifty-five Sprague Dawley rats were randomly divided into normal group(10 rats)and modeling group(45 rats). The rats in the modeling group were induced into diabetic nephropathy model by intraperitoneal injection of streptozotocin. After successful modeling,the rats in the modeling group were randomly divided into model group,Radix Astragali polysaccharide(RAP)group and total saponins of Radix Astragali(TSA) group. Both medication groups were given intragastric administration of corresponding medicine, and the normal group and model group were given intragastric administration of the same volume of distilled water,the treatment lasting 12 weeks. During the medication,the urine volume and 24-hour urinary microalbumin level were dynamically observed every 4 weeks. After 12 weeks of treatment,the ratio of kidney mass to body mass was measured. The levels of renin and angiotensin Ⅱ(AngⅡ)in plasma and renal tissue were detected by radioimmunoassay assay, the mRNA and protein expression levels of angiotensin-converting enzyme(ACE) in renal tissue were separately detected by real-time quantitative polymerase chain reaction(PCR)and immunohistochemistry assay. Results Compared with the normal group,the urine volume,24-hour urinary microalbumin level,ratio of kidney mass to body mass in the model group were increased(P< 0.01),mild proliferative mesangial lesion and increasing extracellular matrix in renal tissue were seen,the levels of renin and AngⅡ in plasma and renal tissue,and the mRNA and protein expression levels of ACE in renal tissue were significantly increased(P< 0.01). Compared with the model group,the urine volume,24-hour urinary microalbumin level,ratio of kidney mass to body mass in the RAP group and TSA group were decreased(P< 0.01),the lesion in renal tissue was improved,the level of AngⅡ in plasma and renal tissue,and the m RNA and p
作者
肖顺强
李佑生
卞伟
蔡晟宇
杨磊
XIAO Shun-Qiang;LI You-Sheng;BIAN Wei;CAI Sheng-Yu;YANG Lei(Shenzhen People’s Hospital/The Second Hospital Affiliated to Jinan University,Shenzhen 518000 Guangdong,China;Luohu District Hospital of Traditional Chinese Medicine/Shenzhen Hospital of Shanghai University of Traditional Chinese Medicine,Shenzhen 518000 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2021年第9期1933-1940,共8页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省基础与应用基础研究基金资助项目(编号:2019A1515110368)。
关键词
黄芪多糖
黄芪总皂苷
糖尿病肾病
肾素-血管紧张素系统
大鼠
Radix Astragali polysaccharide
total saponins of Radix Astragali
diabetic nephropathy
renin angiotensin system
rats
