摘要
目的观察参连复脉颗粒对自发性高血压大鼠(SHR)血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)及心房颤动易感性的影响。方法将60只24周龄的雄性SHR随机分为模型组、氯沙坦钾组(西药组)、参连复脉颗粒低剂量组(低剂量组)、参连复脉颗粒中剂量组(中剂量组)和参连复脉颗粒高剂量组(高剂量组),12只24周龄雄性正常血压WKY大鼠为正常对照组,共6组。给药4周后,酶联免疫吸附测定法(ELISA)测定各组大鼠血清TNF-α、IL-6;多导电生理仪测量P波时限、PR间期、心房有效不应期(AERP)、心房颤动的诱发率(AFI)。结果与正常对照组比较,模型组大鼠血清炎性因子TNF-α、IL-6表达明显增强(P<0.05),P波时限、PR间期、AFI明显增加(P<0.05);与模型组比较,西药组及中剂量组血清TNF-α、IL-6浓度明显降低(P<0.05),低剂量组血清IL-6浓度明显降低(P<0.05),中剂量组、西药组P波时限明显降低(P<0.05),西药组和低剂量组、中剂量组、高剂量组AFI有下降的趋势,但差异无统计学意义(P>0.05),各组AERP及PR间期比较差异无统计学意义(P>0.05)。结论SHR血清炎性因子TNF-α、IL-6升高,心房颤动易感性增加,西药氯沙坦钾片、中药参连复脉颗粒可改善SHR炎症反应,对心房颤动易感性可能有一定抑制作用。
Objective To observe the effect of Shenlian Fumai Granule on serum tumor necrosis factorα(TNF-α),interleukin 6(IL-6),and vulnerability of atrial fibrillation in spontaneously hypertensive rats(SHR).Methods Sixty 24-week-old male SHRs were randomly divided into model group,losartan potassium group(western medicine group),Shenlian Fumai low-dose group(low dose group),Shenlian Fumai medium-dose group(medium dose group)and Shenlian Fumai high-dose group(high dose group).Twelve 24-week-old male WKY rats with normal blood pressure were used as normal control group.Four weeks after intervention,serum TNF-αand IL-6 were detected by enzyme-linked immunosorbent assay(ELISA).The P wave duration,PR interval,atrial effective refractory period(AERP),and the inducibility of atrial fibrillation(AFI)were measured by multi-conductivity physiological apparatus.Results Compared with the normal control group,the expressions of TNF-αand IL-6 in the model group significantly increased(P<0.05),and the P wave duration,PR interval,and AFI significantly increased(P<0.05).Compared with the model group,the expressions of TNF-αand IL-6 in the western medicine group and medium dose group were significantly decreased(P<0.05),the expression of IL-6 in the low dose group significantly decreased(P<0.05),the P wave duration in the western medicine group and medium dose group significantly decreased(P<0.05),the AFI in the western medicine group,low dose group,medium dose group,and high dose group showed a downward trend,but the difference was not statistically significant(P>0.05).There was no statistically significant difference in AERP and PR interval between groups(P>0.05).Conclusion The serum TNF-α,IL-6 and vulnerability of atrial fibrillation in SHR increased.The western medicine losathan potassium tablets and Shenlian Fumai Granules could improve the inflammatory response of SHRs,with a certain inhibitory effect on the vulnerability of atrial fibrillation.
作者
马征
崔晓云
万洁
高群
曲信彦
李星
蔡芸
李岩
林谦
MA Zheng;CUI Xiaoyun;WAN Jie;GAO Qun;QU Xinyan;LI Xing;CAI Yun;LI Yan;LIN Qian(Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中西医结合心脑血管病杂志》
2021年第15期2536-2540,共5页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
国家自然科学基金(No.81874458)
北京中医药大学基本科研业务费项目(No.2019-JYB-XS-218)。
关键词
高血压
炎症
心房颤动
心房颤动易感性
参连复脉颗粒
实验研究
hypertension
inflammation
atrial fibrillation
vulnerability of atrial fibrillation
Shenlian Fumai Granule
experimental study
