摘要
目的建立高效液相色谱(HPLC)法同时测定紫苏子中迷迭香酸、芹菜素和木犀草苷含量的方法。方法色谱柱为Agilent Eclipse C 18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%冰醋酸溶液为流动相,梯度洗脱;流速:1.0 mL·min^(-1),检测波长320 nm;柱温30℃。结果迷迭香酸的含量在0.0583~2.915μg范围内呈良好的线性关系(r=1.0000),平均加样回收率为99.84%,RSD为0.25%(n=6);芹菜素的含量在0.0585~2.925μg范围内呈良好的线性关系(r=1.0000),平均加样回收率为99.83%,RSD为0.77%(n=6);木犀草苷的含量在0.0596~2.980μg范围内呈良好的线性关系(r=1.0000),平均加样回收率为99.39%,RSD为1.07%(n=6)。结论本方法简单快速、灵敏度高、相对更安全,可用于紫苏子中迷迭香酸、芹菜素和木犀草苷含量的同时测定。
Objective To establish a method for simultaneous determination of rosmarinic acid,apigenin and luteolin in Perillae Fructus by HPLC.Methods The three compounds were isolated by using a Agilent Eclipse C 18 chromatographic column(4.6 mm×250 mm,5μm);With methanol-0.1%glacial acetic acid solution as mobile phase,gradient elution was performed.Flow rate:1.0 mL·min^(-1),detection wavelength:320 nm;Column temperature 30℃.Results Rosmarinic acid showed a good linear relationship within the range of 0.0583~2.915μg(r=1.0000),the average recovery was 99.84%,with RSD 0.25%(n=6).Apigenin showed a good linear relationship within the range of 0.0585~2.925μg(r=1.0000),the average recovery was 99.93%,with RSD 0.77%(n=6).Luteolin showed a good linear relationship within the range of 0.0596~2.980μg(r=1.0000),the average recovery was 98.39%,with RSD 1.07%(n=6).Conclusion The method was simple,fast,sensitive and relatively safe,which can be used for simultaneous determination of rosmarinic acid,apigenin and luteolin in Perillae Fructus by HPLC.
作者
权勤波
宋建建
陈淑梅
孙俊英
QUAN Qinbo;SONG Jianjian;CHEN Shumei;SUN Junying(Dongying Center for Food and Drug Control,Dongying 257091,China;Dongying Hekou Administration for Market Regulation,Dongying 257200,China)
出处
《药学研究》
CAS
2021年第6期380-382,396,共4页
Journal of Pharmaceutical Research
