摘要
烟酰胺腺嘌呤二核苷酸(NAD)是所有活细胞中普遍存在的关键辅助分子,在细胞代谢中担任着重要角色。NAD是细胞能量合成过程中的重要因子,参与DNA损伤修复,调控细胞内钙离子浓度等重要生命过程,在细胞的代谢反应中发挥着关键的作用。关于生物细胞内NAD检测的研究一直是近20年生命科学研究中的热点之一。该文从NAD结构特性、合成途径角度着手,总结了国内外关于生物细胞NAD水平检测方法的研究进展,详细介绍了各种方法的原理、优缺点及适用范围并展望了其发展趋势,以期为相关研究提供参考。
Nicotinamide adenine dinucleotide(NAD)is a key accessory molecule prevalent in all liv⁃ing cells and plays an important role in cellular metabolism.NAD directly controls intracellular ener⁃gy metabolism,participates in DNA damage repair,regulates intracellular calcium ion concentra⁃tion,and other important life processes,and plays a critical role in the metabolic reactions of cells.The study of NAD level in biological cells has been one of the research hotspots in life science re⁃search in the last 20 years.In this paper,we summarize the research progress of NAD level detection methods in biological cells from the perspectives of structural properties and synthesis pathways of NAD,introduce the principles,advantages and disadvantages,and the scope of application of vari⁃ous methods,and look forward to the development trend of the methods,in order to provide a good guidance for the related research.
作者
徐涛
金磊
夏鹏志
XU Tao;JIN Lei;XIA Peng-zhi(School of Biological Food and Environment,Hefei University,Hefei 230000,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2024年第7期1076-1085,共10页
Journal of Instrumental Analysis
基金
安徽高校自然科学研究项目(KJ2020A0666)。
