摘要
优化离子液体辅助微波法提取紫苏梗中的黄酮工艺,为进一步利用紫苏梗提供依据。将离子液体加到提取溶剂中,采用辅助微波法提取紫苏梗中的黄酮。依次改变离子液体的种类、浓度、提取溶剂总体积与药材质量比、微波时间、微波温度,以总黄酮提取率为指标,确定单因素最佳条件,在单因素试验结果的基础上结合响应曲面设计优化最佳提取条件,用HPLC测定咖啡酸、木犀草苷、芦丁、迷迭香酸等成分的含量,探究不同方法对紫苏梗中黄酮提取的变化规律。结果表明,最佳提取条件为微波功率750 W,料液比1︰30(g/mL),离子液体体积比12︰1,温度70℃。比较传统方法和高效液相法黄酮中主要成分含量可知,离子液体法比传统方法提取率提高。将离子液体运用于紫苏梗中黄酮提取的方法可行。
The process of extracting flavonoids from perilla stems by ionic liquid-assisted microwave method is optimized to provide a basis for further utilization of perilla stems.Ionic liquids are added to the extraction solvent to assist in the extraction of flavonoids from perilla stems by microwave method.The type and concentration of ionic liquids,the ratio of the total volume of extracted solvent to the mass of medicinal materials,microwave time,and microwave temperature are changed in turn.On the basis of the results of univariate experiments,combined with the design of response surfaces,the optimal extraction condition of single factors is optimized,the optimal conditions of single factors are determined by HPLC,then,the content of caffeic acid,luteolin,rutin,rosmarinic acid and other components is determined by HPLC,and the changes of flavonoid extraction in perilla stems by different methods are explored.The results show that the optimal extraction condition is as follows:microwave power 750 W,material-liquid ratio 1︰30(g/mL),ionic liquid volume ratio 12︰1,and temperature 70℃.Comparing the content of the main components in the traditional method and the high-performance liquid phase method,it can be seen that the extraction rate of ionic liquid method is higher than that of traditional methods.The ionic liquid method used to extract flavonoids from perilla stems is feasible.
作者
闫平
王诗媛
张彦
严惠仲
同红娟
柴希艳
YAN Ping;WANG Shiyuan;ZHANG Yan;YAN Huizhong;TONG Hongjuan;CHAI Xiyan(Xi’an Medical University,Xi’an 710021;Xi’an Quality Life Agricultual Technology Development Industrial Co.,Ltd.,Xi’an 710065;Xi’an Yurun Baide Healthy Management Co.,Ltd.,Xi’an 710065)
出处
《食品工业》
CAS
2024年第2期31-37,共7页
The Food Industry
基金
国家自然科学基金青年项目(81903579)非甾体抗炎药离子液体的结构对其生物利用度的影响
西安市科技局农业技术研发项目(21NYYF062)
西安医学院2021年青年科研基金(2021QN23)
西安医学院科研提升计划项目(2022NLTS082)。
关键词
离子液体
微波
紫苏梗
黄酮
高效液相色谱
ionic liquids
microwave
perilla stems
flavone
high performance liquid chromatography
