摘要
目的检测首发精神分裂症(FES)病人血清中微小RNA-24-2(mir-24-2)、微小RNA-30b(mir-30b)水平,并探讨血清中mir-24-2、mir-30b表达水平与FES发病的关系。方法前瞻性选取2016年11月至2018年9月于武汉市精神卫生中心就诊的98例FES病人为研究对象(FES组);同期选取76例健康体检者作为对照(对照组)。分析两组受试者一般资料;采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测两组受试者血清中mir-24-2、mir-30b表达水平,并对FES病人进行阳性与阴性症状量表(PANSS)评分;分析FES病人血清中mir-24-2、mir-30b水平与PANSS评分相关性;分析血清中mir-24-2、mir-30b水平对FES的诊断价值。结果FES组血清mir-24-2(1.81±0.58)、mir-30b水平(1.46±0.37)、PANSS总评分(78.64±12.31)分、阳性症状评分(12.64±3.73)分、阴性症状评分(20.56±4.32)分、认知评分(8.73±1.84)分、兴奋评分(8.96±2.13)分、抑郁情绪评分(6.75±2.06)分显著高于对照组[(1.00±0.31)、(1.00±0.26)、(35.42±5.37)分、(4.51±1.12)分、(6.94±1.21)分、(3.86±0.95)分、(4.19±0.38)分、(4.12±1.08)分](均P<0.05);FES病人血清中mir-24-2、mir-30b水平与PANSS总评分、阳性症状评分、阴性症状评分、认知评分、兴奋评分、抑郁情绪评分均呈正相关(P<0.05);血清mir-24-2、mir-30b诊断FES的曲线下面积(AUC)分别为0.863(95%CI:0.808~0.919)、0.856(95%CI:0.801~0.911),特异度分别为93.4%、84.2%,灵敏度分别为68.4%、76.5%;两者联合诊断FES的AUC为0.936(95%CI:0.899~0.973),特异度为88.2%,灵敏度为89.8%。结论血清mir-24-2、mir-30b水平在FES病人中明显升高,且与PANSS评分呈正相关。血清mir-24-2、mir-30b可能有助于疾病诊断,对评价精神分裂症的早期危险性有重要意义。
Objective To detect the levels of microRNA-24-2(mir-24-2)and microRNA-30b(mir-30b)in the serum of patients with first-episode schizophrenia(FES),and to explore the relationships between the expressions of mir-24-2 and mir-30b in the serum and the pathogenesis of FES.Methods Ninety-eight FES patients,who were treated in Wuhan Mental Health Center from November 2016 to September 2018,were prospectively selected as the study objects(FES group);76 healthy people were selected as the control group at the same time.The general data of the two groups were analyzed.The expression levels of mir-24-2 and mir-30b in serum of the two groups were detected by real-time fluorescent quantitative PCR(qRT-PCR),and the positive and negative symptom scale(PANSS)scores of FES patients were evaluated.The correlations between the levels of serum mir-24-2 and mir-30b and PANSS were analyzed;and the diagnostic values of mir-24-2 and mir-30b in serum for FES were analyzed.Results The levels of mir-24-2[(1.81±0.58)],mir-30b[(1.46±0.37)],PANSS total score[(78.64±12.31)points],positive symptom score[(12.64±3.73)points],negative symptom score[(20.56±4.32)points],cognitive score[(8.73±1.84)points]、excitement score[(8.96±2.13)points]and depression score[(6.75±2.06)points]in FES group were significantly higher than those in the control group[(1.00±0.31),(1.00±0.26),(35.42±5.37)points,(4.51±1.12)points,(6.94±1.21)points,(3.86±0.95)points,(4.19±0.38)points,(4.12±1.08)points](all P<0.05).The levels of mir-24-2 and mir-30b in FES patients were positively correlated with PANSS total score,positive symptom score,negative symptom score,cognitive score,excitement score and depression score(P<0.05).The area under curve(AUC)of serum mir-24-2 and mir-30b in diagnosing FES were 0.863(95%CI:0.808-0.919)and 0.856(95%CI:0.801-0.911).The specificity were 93.4%and 84.2%,the sensitivity were 68.4%and 76.5%,respectively.The AUC of the combination in diagnosing FES was 0.936(95%CI:0.899-0.973),the specificity was88.2%,and the sensitivity was 89.8%
作者
雷雨
刘伟
包黎
LEI Yu;LIU Wei;BAO Li(Department of Psychiatry,Wuhan Mental Health Center,Wuhan,Hubei 430022,China)
出处
《安徽医药》
CAS
2021年第6期1204-1207,共4页
Anhui Medical and Pharmaceutical Journal
