摘要
为明确放养条件下红河黄牛的营养和免疫状况,选取60头红河黄牛,公母各半,按性别和年龄(12、36和60月龄)分成6个组,颈静脉采血分离血清,测定生化和免疫指标。结果显示:①相同性别的黄牛血清总蛋白(TP)和球蛋白(GLOB)含量12月龄显著低于60月龄(P<0.05),母牛碱性磷酸酶(ALP)活性和血糖(GLU)含量12月龄显著高于60月龄(P<0.05),其他血清生化指标在不同月龄间均无显著差异(P>0.05)。公、母牛血清TP和GLOB含量均随月龄增加而升高,而ALP活性随月龄增加而降低。不同月龄母牛血清TP、白蛋白(ALB)、GLOB含量和谷丙转氨酶(ALT)活性均高于公牛(P<0.05);而ALP活性和GLU含量公牛高于母牛(P<0.05)。②除母牛血清IL-8在12和36月龄间存在显著差异(P<0.05)外,相同性别的黄牛血清白细胞介素(IL)-2、IL-8、IL-10、免疫球蛋白(Ig)A、IgG、IgM、γ干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)含量在3个月龄间均无显著差异(P>0.05)。不同月龄母牛IL-2、IgA、IgG、IgM、IFN-γ和TNF-α含量均高于公牛(P<0.05)。综合以上结果,红河黄牛公、母牛随月龄增加,血清TP和GLOB含量显著增加,而ALP活性降低;母牛血清生化和免疫指标总体高于公牛。
To clarify the nutritional and immune status of Honghe Yellow cattle under the grazing condition,60 Honghe Yellow cattles comprising half males and half females were selected and divided into 6 groups according to sex and age(12,36 and 60 months old).Their sera were collected from jugular vein to determine biochemical and immune indicators.As a result,①There were significantly lower contents of total proteins(TP)and globulin(GLOB)within the same sex at the age of 12 months than those at the age of 60 months(P<0.05);The activity of alkaline phosphatase(ALP)and the content of glucose(GLU)were significantly higher in 12-month-old cattle than in 60-month-old cattle within the female(P<0.05),and no significant difference existed for the other serum biochemical indicators between ages.With the increase of age,the contents of TP and GLOB increased while the average activity of ALP decreased in the sera of both males and females.The contents of TP,albumin(ALB),GLOB and aspartate aminotransferase(AST)of females at the three ages were higher than those of males,but the activity of ALP and the content of GLU of males were higher than those of females.②For the same sex,the serum levels of interleukin(IL)-2,IL-8,IL-10,immunoglobulin(Ig)A,IgG,IgM,interferon-γ(IFN-γ),and tumor necrosis factorα(TNF-α)were not significantly different among the three ages(P>0.05),except for a significant difference in serum IL-8 between 12 and 36-month females(P<0.05).The contents of IL-2,IgA,IgG,IgM,IFN-γand TNF-αin 12,36 and 60-month-old females were higher than those in males(P<0.05).In conclusion,with the increase of age,the serum TP and GLOB contents significantly increased while the ALP activity decreased,and basically,females had higher serum biochemical indexes and immune indexes than females in Honghe Yellow cattle.
作者
鲁琼芬
陶杨
盖叶顶
李鹏飞
杨仁辉
余烨
付斌龙
李晓峰
杨红玖
白荣芳
杨志新
冷静
LU Qiongfen;TAO Yang;GAI Yeding;LI Pengfei;YANG Renhui;YU Ye;FU Binlong;LI Xiaofeng;YANG Hongjiu;BAI Rongfang;YANG Zhixin;LENG Jing(Yunnan Provincial Key Laboratory of Animal Nutrition and Feed,Yunnan Agricultural University,Kunming 650201,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yisa Town Agricultural Comprehensive Service Center,Honghe County,Honghe 654400,China;Honghe County Animal Husbandry Technology Extension Station,Honghe 654400,China)
出处
《中国畜牧兽医》
CAS
北大核心
2021年第5期1574-1583,共10页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31672452)
云岭产业技术领军人才项目
云南省现代农业产业技术体系项目(2017KJTX0016-5)
云南省科技厅重点研发计划(2018BB001-2)。
关键词
红河黄牛
生化指标
免疫指标
Honghe Yellow cattle
biochemical indexes
immune indexes
