摘要
【目的】天蚕素抗菌肽是一种商品化的阳离子抗菌肽,在水貂体内的研究较少。试验旨在探究抗菌肽对育成期母貂脏器系数、血清及空肠免疫指标、肠组织形态的影响。【方法】选取65日龄体重相近的短毛黑母貂60只,随机分为6组,每组10个重复,每个重复1只,单笼饲养。各组饲粮中分别添加0(对照组)、100、200、300、400和500 mg/kg抗菌肽,预试期1周,正试期8周。饲养试验结束后,每组取6只水貂进行心脏采血,采集内脏器官、十二指肠及空肠样品,并测定血清及空肠免疫指标、肠道组织形态。【结果】与对照组相比,100、300和400 mg/kg抗菌肽组脾脏指数显著升高(P<0.05);各试验组补体C3(C3)、补体C4(C4)和免疫球蛋白M(IgM)含量均显著升高,除200 mg/kg抗菌肽组外的所有试验组免疫球蛋白A(IgA)和免疫球蛋白G(IgG)含量均显著升高(P<0.05);各试验组肿瘤坏死因子(TNF-α)含量均显著降低,100、200、300 mg/kg抗菌肽组分泌型免疫球蛋白(sIgA)、γ干扰素(IFN-γ)及白细胞介素-10(IL-10)含量均显著升高(P<0.05);各试验组十二指肠和空肠绒毛高度均显著升高,且200 mg/kg抗菌肽组十二指肠隐窝深度显著低于400和500 mg/kg抗菌肽组,而绒毛高度/隐窝深度(V/C)显著高于对照组、400和500 mg/kg组(P<0.05);200、300和400 mg/kg抗菌肽组空肠V/C均显著升高(P<0.05)。【结论】饲粮中添加天蚕素抗菌肽可促进育成期母貂免疫器官发育,提高机体免疫力,改善肠组织形态,建议抗菌肽添加水平为100~200 mg/kg。
【Objective】Cecropin antimicrobial peptide is a commercial cationic antimicrobial peptide that has been seldom studied in minks.The experiment was conducted to determine the effects of antimicrobial peptide on organ coefficient,serum and jejunum immune indexes and intestinal tissue morphology of growing female minks.【Method】A total of 60 healthy 65-day-old short-haired black female minks with similar body weight were selected and randomly divided into 6 groups with 10 replicates in each group and one mink in each replicate.Minks were separately fed diets supplemented with 0(control group),100,200,300,400 and 500 mg/kg antimicrobial peptide,respectively.The pre-experimental period lasted for 1 week,and the experimental period lasted for 8 weeks.After the feeding experiment,6 minks from each group were selected to collect samples of blood,internal organs,duodenum and jejunum,the immune indicators and intestinal tissue morphology were analyzed.【Result】Compared with control group,the spleen indexes of 100,300 and 400 mg/kg antimicrobial peptide groups significantly increased(P<0.05).The contents of C3,C4 and IgM in experimental groups,and the contents of IgA and IgG in experimental groups(except for 200 mg/kg antimicrobial peptide group)were significantly increased(P<0.05).The contents of TNF-αin experimental groups were significantly decreased,and the contents of sIgA,IFN-γand IL-10 in 100,200 and 300 mg/kg groups were significantly increased(P<0.05).The villus heights of duodenum and jejunum in experimental groups were significantly increased(P<0.05),the crypt depth of duodenum in 200 mg/kg antimicrobial peptide group was significantly lower than that in 400 and 500 mg/kg antimicrobial peptide groups,and V/C in 200 mg/kg antimicrobial peptide group was significantly higher than that in control,400 and 500 mg/kg antimicrobial peptide groups(P<0.05).V/C of jejunal in 200,300 and 400 mg/kg antimicrobial peptide groups were significantly increased(P<0.05).【Conclusion】In conclusion,antimicrobial peptide
作者
宇晓军
李丹丹
厉学武
王群
王光
王利华
YU Xiaojun;LI Dandan;LI Xuewu;WANG Qun;WANG Guang;WANG Lihua(College of Animal Science and Technology, Qingdao Agricultural University,Qingdao266109,China;Shandong Newhope Liuhe Group Co.,Ltd.,Qingdao 266000, China)
出处
《中国畜牧兽医》
CAS
北大核心
2022年第2期443-453,共11页
China Animal Husbandry & Veterinary Medicine
基金
山东省现代农业产业技术体系特种经济动物创新团队(SDAIT-21-8)。
关键词
抗菌肽
水貂
脏器系数
免疫功能
肠组织形态
antimicrobial peptide
mink
organ coefficient
immune function
intestinal tissue morphology
