摘要
以煤矸石山种植年限为17年的香根草Vetiveria zizanioides为研究对象,采用高通量测序技术研究香根草根系及根际土丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)群落的季节动态。并利用Spearman、NMDS、RDA和Mantel-test等统计学方法分析了AMF孢子密度、菌根侵染率、土壤理化性质、丰富度及多样性指数之间的相关性。结果表明:(1)AMF孢子密度和菌根侵染率均表现为夏季显著高于春秋两季。(2)3个季节根系中共得到127个OTUs,根际土中共得到258个OTUs,根和根际土中共享96个OTUs,共分离鉴定4科7属AMF,球囊霉属Glomus是该煤矸石山的优势属。(3)根系中AMF多样性和丰富度指数随季节变化先降后升;根际土中AMF多样性指数随季节变化先降后升,但丰富度指数随季节变化呈递减趋势;春季根际土香农、Chaol和ACE指数均显著高于根系,夏季根际土Chaol指数显著高于根系。(4)NMDS分析表明,夏季根系AMF群落分布与其他季节差异显著。Spearman分析表明,根系全钾含量与多样性和丰富度指数显著正相关,速效磷与丰富度指数显著负相关。RDA分析表明,AMF群落组成在根系春季和夏季、根际土夏季和秋季与全磷、全氮、全钾和速效磷正相关,与有机质负相关。Mantel-test分析表明,pH、有机质、全氮、全钾、速效磷和采样季节极显著影响菌根侵染率,有机质和全氮是影响孢子密度的主导因子。
The high-throughput sequencing was used to determine the seasonal dynamics of community of arbuscular mycorrhizal fungi(AMF)in the root and rhizosphere soil of Vetiveria zizanioides in coal gangue dump with planting period of 17 years.Spearman,NMDS,RDA and Mantel test were used to analyze the correlation among AMF spore density,colonization rate,physicochemical properties of soil,richness and diversity index.The results showed that the spore density and colonization rate of AMF in summer were significantly higher than those in spring and autumn.In spring,summer and autumn,127 OTUs,258 OTUs and 96 OTUs were obtained from root system,rhizosphere soil and both of them,respectively,and 7 genera and 4 families of AMF were identified.Glomus was the dominant genus in the coal gangue dump.The AMF diversity and richness index of root first decreased and then increased with the seasonal change;the AMF diversity index of rhizosphere soil first decreased and then increased,while the richness index showed a decreasing trend with the seasonal change.Shannon,Chao1 and ACE indexes of rhizosphere soil in spring were significantly higher than those of root system,and the Chaol index of rhizosphere soil was significantly higher than that of root in summer.NMDS analysis showed that the distribution of AMF community in summer was significantly different from that in other seasons.Spearman analysis showed that total potassium content in roots was significantly positively correlated with diversity and richness index of AMF.There was a significant negative correlation between available phosphorus and AMF richness index.The results of RDA analysis showed that AMF community composition was positively correlated with total phosphorus,total nitrogen,total potassium and available phosphorus in root system in spring and summer and in rhizosphere soil in summer and autumn,but it was negatively correlated with organic matter.Mantel test analysis showed that pH,organic matter,total nitrogen,total potassium,available phosphorus and sampling seaso
作者
王化秋
程巍
郝俊
毛圆圆
卢绮
古天岳
李栋恒
WANG Hua-Qiu;CHENG Wei;HAO Jun;MAO Yuan-Yuan;LU Qi;GU Tian-Yue;LI Dong-Heng(Department of Grassland Science,College of Animal Science,Guizhou University,Guiyang,Guizhou 550025,China;Key Laboratory of Mountain Plant Resources Protection and Germplasm Innovation,Ministry of Education,Guiyang,Guizhou 550025,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第3期514-530,共17页
Mycosystema
基金
国家自然科学基金(31500451)
贵州省科技计划项目(黔科合支撑字[2020]1Y046号)。
关键词
香根草
多样性
丰富度
AMF群落
季节动态
煤矸石山
Vetiveria zizanioides
diversity
richness
AMF community
seasonal dynamic
coal gangue dump
