摘要
采用传统染色检测与PCR-DGGE相结合的方法,研究了柠条根系丛枝菌根真菌(AMF)的季节性变化,并利用主成分分析(PCA)和典范对应分析(CCA)分析了土壤因子与AMF季节变化之间的关系.结果表明:柠条根系AMF总侵染率、丛枝侵染率、泡囊侵染率和土壤孢子密度均具有显著的季节性差异.总侵染率从春季至秋季呈下降趋势,其他指标呈上升趋势.春、夏、秋3个季节的柠条根系中共检测出9种AMF分子种,但每个季节的AMF群落组成、多样性指数均有一定的差异.主成分(PCA)和典范对应分析(CCA)表明,随着土壤总氮、总磷和有机碳的增加,泡囊侵染率减少;随着土壤含水量和速效磷的降低,土壤AMF孢子密度增加;根系内AMF群落组成的季节性差异主要受土壤总磷、速效磷和有机碳的影响.
With the combination of root staining and PCR-DGGE, the seasonal variation of arbuscular mycorrhizal fungi （AMF） in Caragana korshinskii roots was investigated; and by the methods of principal component analysis and canonical correspondence analysis, the relationships between this variation and soil factors were analyzed. It was found that the total infection rate of AMF and the infection rates of arbuscules and vesicles in C. korshinskii roots as well as the spore density of AMF in soil varied significantly among seasons. From spring to autumn, the total infection rate of AMF had a decreasing trend, whereas the other three indicators were in advers,e. A total of nine AMF phylotypes were detected in spring, summer, and autumn, but the community composition and diversity indices of AMF had definite differences among the seasons. The infection rate of vesicles decreased with increasing soil total N, P, and organic C, while the spore density of AMF increased with decreasing soil moisture and available P. The seasonal variation of AMF community composition in C. korshinskii roots was mainly affected by soil total P, available P, and organic C.
出处
《应用生态学报》
CAS
CSCD
北大核心
2009年第5期1085-1091,共7页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30570270
30870438)
教育部留学回国人员启动基金项目
教育部新世纪人才计划项目(NCET-07-0390)
高等学校博士点基金项目(20070730013)
西北民族大学中青年科研基金项目(XBMU-2007-BD-36)资助.
关键词
丛枝菌根
柠条
季节性
土壤性质
arbuscular mycorrhiza
Caragana korshinskii
seasonality
soil property.
