摘要
【目的】脐橙的养分与水分吸收对丛枝菌根真菌(AM真菌)的依赖度非常高,而AM真菌的群落结构和多样性决定着生态系统功能和脐橙产量。全国最大的脐橙主产区—江西省赣州市,被誉为“中国脐橙之乡”。但关于赣南脐橙根际土AM真菌的分子多样性研究较为欠缺,且是否存在地域差异尚需明确。研究野外红肉脐橙根际AM真菌群落特征,探究其地域差异,为研制脐橙高效促生AM真菌菌肥,促进脐橙产业的发展提供科学指导。【方法】在章贡区、会昌县和信丰县3个赣南脐橙主栽培区,采集红肉脐橙根际土样,利用Illumina MiSeq高通量测序技术结合DADA2算法调查3个地域红肉脐橙根际土AM真菌物种组成、群落结构和多样性,研究其区域差异,并采用常规方法测定土壤化学性质,利用冗余分析和相关性分析研究了土壤化学因子与AM真菌群落特征的关系。【结果】3个地域红肉脐橙根际土壤化学性质除碱解氮含量外,差异均较小;红肉脐橙根际土共得到90437条AM真菌基因序列,186种AM真菌ASVs,属于1门、3纲、3目、4科、4属、16种,其中,球囊霉属Glomus(相对丰度97.70%)为绝对优势属,Glomus_sp(相对丰度18.52%)为优势种;红肉脐橙根际土AM真菌群落α多样性(ASV数量、Shannon指数和Pielous指数)具有显著地域差异,信丰县Shannon指数显著高于章贡区和会昌县(P<0.05),ASV数量显著高于会昌县(P<0.05),Pielous指数显著高于章贡区(P<0.05),而AM真菌群落结构无显著差异;3个地域红肉脐橙根际土中优势菌属Glomus相对丰度排序为章贡区>会昌县>信丰县,而优势菌种Glomus_sp排序为会昌县>章贡区>信丰县;菌属相对丰度地域间无显著差异(P>0.05),而菌种Glomus_Toljander08-Unk1_VTX00222在信丰县红肉脐橙根际土的相对丰度显著高于其他两个地域(P<0.05);冗余分析表明,土壤有机质是影响红肉脐橙根际土AM真菌多样性的最主要土壤因子,贡献�
【Objectives】The nutrient and water absorption of navel orange are highly dependent on arbuscular mycorrhizal fungi(AM fungi).The community structure and diversity of AM fungi determine the function of ecosystem and navel orange yield.Ganzhou City of Jiangxi Province is the largest navel orange production area in China,known as the“hometown of navel oranges in China”.We studied the AM fungal community characteristics in the main navel orange production areas,so as to provide scientific guidance for the development of AM fungal fertilizer and navel orange industry.【Methods】Rhizosphere soil samples were collected in Zhanggong District,Huichang County,and Xinfeng County,the three tyoical navel orange(Citrus sinensis Osbeck cv.Cara)production areas.Illumina MiSeq high-throughput sequencing technology combined with DADA2 algorithm were used to analyze the AM fungal community.Conventional chemical methods were used to analyze the soil chemical properties.Redundancy analysis(RDA)and correlation analysis were used to determine the relationships between AM fungal community and soil properties.【Results】The rhizosphere soil chemical properties in the 3 regions were not significantly different,except for alkali-hydrolyzed nitrogen content.A total of 90437 genetic sequences and 186 ASVs were obtained from the rhizosphere soils of navel orange,belonging to 1 phylum,3 class,3 orders,4 family,4 genera,and 16 species.Among them,Glomus was the absolutely dominant genus,with a relative abundance of 97.70%,while Glomus_sp was the dominant genus,with a relative abundance of 18.52%.Theαdiversities(ASV number,Shannon index,and Pielous index)of AM fungal communities were significantly different among the three regions.In Xinfeng County,the Shannon index was higher than that in Zhanggong District and Huichang County(P<0.05),the ASV number was higher than that in Huichang County(P<0.05),while the Pielous index was higher than that in Zhanggong District(P<0.05).The AM fungal community structure did not show significant regi
作者
杨梦佳
邹丛荣
王金平
黄荣珍
姬绍晖
谢荣秀
房焕英
黄国敏
YANG Meng-jia;ZOU Cong-rong;WANG Jin-ping;HUANG Rong-zhen;JI Shao-hui;XIE Rong-xiu;FANG Huan-ying;HUANG Guo-min(Jiangxi Provincial Technology Innovation Center for Ecological Water Engineering in Poyang Lake Basin,Nanchang Institute of Technology,Nanchang,Jiangxi 330099,China;Yichun Forestry Bureau,Yichun City,Yichun,Jiangxi 336099,China)
出处
《植物营养与肥料学报》
CAS
CSCD
北大核心
2023年第8期1520-1530,共11页
Journal of Plant Nutrition and Fertilizers
基金
江西省教育厅科学技术研究项目(GJJ201924)
江西省自然科学基金项目(20224BAB215032)
国家自然科学基金项目(31901091)。
关键词
脐橙
AM真菌
群落多样性
高通量测序
土壤因子
navel orange
AM fungi
community diversity
high-throughput sequencing
soil factor
