摘要
【目的】探究杨树Populus spp.人工林土壤丛枝菌根(arbuscular mycorrhizal,AM)真菌群落结构和多样性对氮添加的季节动态响应及其驱动因素。【方法】以江苏省东台林场杨树为对象,研究5种氮添加水平(0、5、10、15和30g·m^(-2)·a^(-1))处理6 a后土壤理化性质、AM真菌群落结构和多样性随季节的动态变化,并分析了AM真菌群落特征与土壤环境因子的关系。【结果】(1)高通量测序共获得AM真菌有效序列1307513条,196个分类操作单元(OTU)分属于4目8科10属,其中球囊霉属Glomus和多胞囊霉属Diversispora相对丰度之和平均达99.3%;(2)氮添加处理对AM真菌多样性指数影响不显著,但随着氮添加水平的增加多胞囊霉属的相对丰度逐渐降低,其中高氮(30 g·m^(-2)·a^(-1))处理球囊霉属的相对丰度显著(P<0.05)高于低氮处理(5和10 g·m^(-2)·a^(-1));(3)季节对AM真菌群落结构(基于OTUs,r=0.695)及Chao指数、Simpson指数均有显著影响,且秋季和春季的AM真菌多样性指数均显著高于夏季(P<0.01);(4)冗余分析结果表明:土壤pH、总磷和总氮等9个环境因子对AM真菌群落组成变化的解释量为57.6%(P=0.001),其中AM真菌群落组成与土壤温度的相关性最大(r=0.766),另外群落组成和多样性指数均与pH、总磷、总碳和铵态氮有显著相关关系(P<0.05)。【结论】氮添加下,杨树人工林土壤AM真菌群落具有季节性响应,土壤温度、pH、总磷、总碳和铵态氮是影响AM真菌群落动态变化的主要驱动因素。
[Objective]The study aimed to investigate the effects of nitrogen addition and sampling season on soil arbuscular mycorrhizal(AM)fungal community structure and diversity in a poplar plantation,and clarify the driving factors.[Method]A field simulating experiment with five nitrogen addition levels(0,5,10,15 and 30 gm^(-2)·a^(-1))was conducted in a poplar plantation in Dongtai Forest Farm,Jiangsu.After six years of continuous nitrogen additions,the dynamic changes of soil physicochemical properties,AM fungal community diversity and composition in four seasons(spring,summer,autumn and winter)were examined,and the relationships between the characteristics of AM fungal community and soil environmental factors were also analyzed.[Result](1)A total of 1307513 high-quality AM fungal sequences were identified by high-throughput sequencing,and they were assigned to 196 OTUs,4 orders,8 families,and 10 genera.The dominated genera were Glomus and Diversispora,and the total average relative abundances of them reached to 99.3%.(2)Nitrogen addition had no significant effect on the AM fungal community structure and diversity.However,the relative abundance of Diversispora decreased significantly with the increase of nitrogen addition level,and the relative abundances of Glomus under high nitrogen addition(30 gm^(-2)·a^(-1))was significantly higher than under low nitrogen additions(5 and 10 gm^(-2)·a^(-1))(P<0.05).(3)Season had significant effects on the AM fungal community Chao index,Simpson index and community structure(based on OTUs,r=0.695),and the AM fungal community diversity indexes in autumn and spring were significantly higher than that in summer(P<0.01).(4)Redundancy analysis showed that nine environmental factors measured in this study explained 57.6%variance in the AM fungal community composition,passing the Monte Carlo test with 999 permutations(P=0.001).Among these factors,temperature had the greatest correlation with the AM fungal community composition(r=0.766).Moreover,pH,total P,total C and NH4+-N were significa
作者
彭思利
张鑫
武仁杰
蔡延江
邢玮
葛之葳
毛岭峰
PENG Sili;ZHANG Xin;WU Renjie;CAI Yanjiang;XING Wei;GE Zhiwei;MAO Lingfeng(Co-Innovation Center for Sustainable Forestry in Southern China,College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;College of Environment and Resources,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Jiangsu Academy of Forestry Sciences,Nanjing 211153,Jiangsu,China;Yancheng Coastal Wetland Ecosystem National Research Station,Jiangsu Academy of Forestry Sciences,Yancheng 224000,Jiangsu,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2023年第4期792-800,共9页
Journal of Zhejiang A&F University
基金
江苏省2022年度碳达峰碳中和科技创新专项资金项目(BE2022305)
江苏省科技厅社会发展面上项目(BE2022792)
江苏省林业科技创新与推广项目(LYKJ[2021]25)
国家自然科学基金资助项目(41601254,32271712)。
关键词
氮沉降
季节动态
丛枝菌根真菌
群落结构
多样性
杨树人工林
nitrogen deposition
seasonal dynamics
arbuscular mycorrhizal fungi
community composition
diversity
poplar plantation
