摘要
目的探究水通道蛋白-4(AQP-4)、血管细胞粘附分子-1(VCAM-1)、神经肽Y(NPY)在重症手足口病(HFMD)患儿脑脊液中的表达情况,并探究其对并发脑炎的诊断价值。方法选取本院36例重症HFMD患儿作为重症组,另选取32例普通HFMD患儿作为普通组。检测对比两组、重症组并发与未并发脑炎患儿脑脊液AQP-4、VCAM-1、NPY水平,分析3者与病情程度的关系,探究脑脊液AQP-4、VCAM-1、NPY对重症HFMD并发脑炎的诊断价值。结果重症组脑脊液AQP-4、VCAM-1、NPY水平高于普通组,差异有统计学意义(P<0.05);HFMD病情程度与脑脊液AQP-4、VCAM-1、NPY水平显著相关,差异有统计学意义(P<0.05);重症组并发脑炎患儿脑脊液AQP-4、VCAM-1、NPY水平高于未并发脑炎患儿,差异有统计学意义(P<0.05);脑脊液AQP-4、VCAM-1、NPY联合诊断重症HFMD并发脑炎的AUC为0.894,大于各指标单独诊断,联合诊断的最佳敏感度为81.25%,特异度为90.00%。结论脑脊液AQP-4、VCAM-1、NPY表达水平在重症HFMD并发脑炎诊断方面具有较高应用价值,可为临床评估HFMD病情程度、诊断重症HFMD并发脑炎提供重要参考。
Objective To explore the expression of aquaporin-4(AQP-4),vascular cell adhesion molecule-1(VCAM-1),neuropetide Y(NPY)in the cerebrospinal fluid of children with severe hand-footmouth disease(HFMD),and their diagnostic value for complicated encephalitis. Methods 36 children with severe HFMD in our hospital were selected as the severe group,and 32 children with ordinary HFMD as the normal group. The levels of AQP-4,VCAM-1,and NPY in the cerebrospinal fluid of children with and without encephalitis were compared between the two groups and the severe group,and the relationship between the three and the severity of the disease was analyzed. The value of cerebrospinal fluid AQP-4,VCAM-1,NPY in the diagnosis of severe HFMD complicated with encephalitis was explored. Results The levels of AQP-4,VCAM-1 and NPY in the cerebrospinal fluid of the severe group were higher than those in the normal group,and the difference was statistically significant(P<0.05). The severity of HFMD was significantly correlated with the levels of AQP-4,VCAM-1,and NPY in cerebrospinal fluid,and the difference was statistically significant(P<0.05).The levels of AQP-4,VCAM-1 and NPY in cerebrospinal fluid of children with severe encephalitis were higher than those of children without encephalitis,and the difference was statistically significant(P<0.05);the AUC of AQP-4,VCAM-1,and NPY in cerebrospinal fluid combined diagnosis of severe HFMD complicated with encephalitis was 0.894,which was greater than each index alone,the best sensitivity of the combined diagnosis was 81.25%,and the specificity was 90.00%. Conclusion The abnormal increase in the expression levels of AQP-4,VCAM-1 and NPY in high application value in the diagnosis of severe HFMD complicated with encephalitis. It can be used for clinical evaluation of the severity of HFMD and diagnosis of severe disease.
作者
杜伟鹏
汪海霞
卢芳
DU Weipeng;WANG Haixia;LU Fang(Department of Medical Laboratory,Nanyang Central Hospital,Nanyang,Henan,China,473009)
出处
《分子诊断与治疗杂志》
2021年第3期352-355,共4页
Journal of Molecular Diagnostics and Therapy
基金
河南省科技厅基金项目(20190038x)。
关键词
重症手足口病
脑脊液
水通道蛋白-4
血管细胞粘附分子-1
神经肽Y
脑炎
Severe hand-foot-and-mouth disease
Cerebrospinal fluid
Aquaporin-4
Vascular cell adhesion molecule-1
Neuropeptide Y
Encephalitis
