摘要
目的探讨脑胶质瘤向周围正常脑组织的侵袭生长与细胞间黏附分子-1(ICAM-1)及血管-细胞黏附分子-1(VCAM-1)的关系。方法共收集44例脑胶质瘤病例,取瘤周组织、肿瘤中心和正常脑组织标本,分别用免疫组化(IHC)、反转录PCR(RT-PCR)和蛋白免疫印迹(Western blot)法检测ICAM-1及VCAM-1在3组标本中的表达量。结果瘤周组织、肿瘤中心组织及正常脑组织比较ICAM-1及VCAM-1表达有显著差异(P<0.05);当进行组间两两比较时有显著差异(P<0.01),瘤周组织中表达量最高,肿瘤中心组织次之,正常脑组织最少,将标本分为LGGs组和HGGs组后进行比较可得出同样结论。结论 ICAM-1和VCAM-1与脑胶质瘤向周围正常脑组织的侵袭生长有密切关系。
Objective To explore the relationship between ICAM-1,VCAM-1 and invasibility of glioma. Methods The peripheral,central part of glioma and the normal brain tissue were obtained from 44 specimens with glioma.The expression of ICAM-1 and VCAM-1 in these specimens were detected by immunohistochemistry（ IHC）,RTPCR and Western blot. Results The difference between peripheral,central part of glioma and normal brain tissue are statistically significant（ P〈 0. 05）. The difference among these three groups were statistically significant（ P〈 0. 01）. The expression in tumor peripheral tissue is the highest,followed by the tumor central tissue,and the expression in normal brain tissue is the lowest. The result is the same as above when these specimens were grouped by pathological grade. Conclusions ICAM-1 and VCAM-1 have significant relation to invasibility of glioma.
出处
《基础医学与临床》
CSCD
2016年第12期1703-1707,共5页
Basic and Clinical Medicine
