摘要
以刺梨果渣为原料,通过SDS-超声辅助的方法,用水和乙醇两种溶剂分别提取总黄酮。在单因素实验基础上,依据Box-Behnken原理优化不同提取方式下刺梨果渣黄酮提取的最优工艺条件。结果表明,以SDS-超声辅助水提取的方法在其最佳工艺参数为料液比1∶47(g/mL)、SDS浓度1.26%、超声时间45 min的条件下获得的刺梨果渣黄酮实际萃取率最高,为0.699%,与预测萃取率0.711%之间的相对误差为1.72%。而SDS-超声辅助醇提取和未加SDS的醇提取两种方法在各自的最优提取条件下获得的黄酮实际萃取率分别为0.448%和0.419%。说明SDS-超声辅助水提取的方法更适合刺梨果渣中黄酮的提取,且方法准确可靠。
Total flavonoids from the pomace of Rosa roxburghii Tratt.were extracted by SDS-ultrasound-assisted method with water and ethanol,respectively.Based on single-factor experiments and the Box-Behnken principle,extraction conditions under different extraction methods were optimized.Actual yield of flavonoids was the highest,0.699%,and the relative error of the predicted yield 0.711% was 1.72%,under the optimum technological parameters of water extraction as follows:Solid-liquid ratio 1∶47(g/mL),SDS concentration 1.26%,and ultrasonic time 45 min.However,the actual yields of flavonoids obtained by SDS-ultrasonic-assisted alcohol extraction and SDS-free alcohol extraction under the respective optimal extraction conditions were 0.448% and 0.419%,respectively.SDS-ultrasound-assisted water extraction was more suitable for extracting flavonoids from Rosa roxburghii Tratt.pomace,and the method was accurate and reliable.
作者
王绪英
赵文
杨洪
廖雯
向红
朱淼
WANG Xu-ying;ZHAO Wen;YANG Hong;LIAO Wen;XIANG Hong;ZHU Miao(School of Biological Science and Technology,Liupanshui Normal University,Liupanshui 553000,China)
出处
《化学试剂》
CAS
北大核心
2021年第3期383-389,共7页
Chemical Reagents
基金
六盘水市科技局项目(52020-2019-05-02)
贵州省科技厅基金资助项目(黔科合LH字[2015]7609号)
贵州省教育厅基金资助项目(黔教合KY字[2017]012号)
贵州省教育厅基金资助项目(黔教合KY字[2019]135)。
关键词
刺梨果渣
黄酮
SDS-超声辅助提取
响应面法
the pomace of Rosa roxburghii Tratt.
flavonoids
SDS-ultrasonic-assisted extraction
response surface methodology
