摘要
目的:在“成分-功效”关联模式下,探究补骨脂四种不同浓度乙醇提取物主要活性成分含量、抗氧化及胆酸盐结合能力差异。方法:以补骨脂为原料,采用无水乙醇、75%乙醇、50%乙醇、水进行提取,比较其总黄酮和总多酚含量,并分别通过3种抗氧化体系与胆酸盐结合能力进行体外抗氧化和降脂活性评价,并进行相关性分析。结果:无水乙醇提取物总黄酮含量(50.317±0.018 mg/g)和总多酚含量(3.860±0.045 mg/g)最高。补骨脂50%乙醇提取物对ABTS^(+)·和DPPH·清除能力最强,IC_(50)值分别为0.76和1.63 mg/mL,无水乙醇提取物还原能力最强,A0.5值为2.63 mg/mL。补骨脂水提物对胆酸钠的结合能力最强,IC_(50)值为0.67 mg/mL,而50%乙醇提取物对牛磺胆酸钠和甘胺胆酸钠结合能力最强,IC_(50)值分别为1.26和0.41 mg/mL。相关性结果表明,补骨脂不同浓度乙醇提取物中总黄酮含量与DPPH·清除率、牛磺胆酸钠结合率呈显著正相关性(P<0.05),总黄酮、总多酚含量与还原能力均呈极显著性正相关关系(P<0.01),而总多酚含量与胆酸钠结合率呈显著性负相关性(P<0.05)。结论:补骨脂50%乙醇提取物活性含量较高,抗氧化及结合胆酸盐能力最强,可以作为潜在的天然抗氧化及降脂活性物质的重要来源。
Objective:The contents of main active ingredients,antioxidant and cholate binding ability of different ethanol extracts from Psoralea corylifolia L.were explored based on the mode of correlation of“components-efficacy”.Methods:Psoralea corylifolia L.was extracted with anhydrous ethanol,75%ethanol,50%ethanol and water.The contents of total flavonoids and total polyphenols in Psoralea corylifolia L.were determined.The antioxidant and lipid-lowering activities in vitro of Psoralea corylifolia L.were evaluated by three antioxidant systems and the binding ability of cholate salts,respectively,and the correlation analysis was conducted.Results:The contents of total flavonoids and total polyphenols in anhydrous ethanol extract were the highest,(50.317±0.018)mg/g and(3.860±0.045)mg/g,respectively.The 50%ethanol extract showed the best ability to scavenge ABTS^(+)·and DPPH·,the IC_(50) values were 0.76 and 1.63 mg/mL,respectively.The anhydrous ethanol extract showed the strongest reducing ability,the A0.5 value was 2.63 mg/mL.The water extract showed the strongest binding capacity to sodium cholate,the IC_(50) value was 0.67 mg/mL.The 50%ethanol extract showed the strongest binding capacity to sodium taurocholate and sodium glycinate,the IC_(50) values were 1.26 and 0.41 mg/mL,respectively.The correlation results showed that the content of total flavonoids of different ethanol extracts from Psoralea corylifolia L.had a significant positive correlation with DPPH·clearance rate and sodium taurocholate binding rate(P<0.05).And the content of total flavonoids and total polyphenols had a very significant positive correlation with reduction ability(P<0.01).While the content of total polyphenols had a significant negative correlation with sodium cholate binding rate(P<0.05).Conclusion:The 50%ethanol extract of Psoralea corylifolia L.has high content of active ingredients and the strongest antioxidant and cholate binding ability.It can be used as a potential important source of natural antioxidant and lipid-lowering acti
作者
姜欣洋
梁金月
史继童
刘兰玲
张珊珊
赵盼
颜培正
赵东升
JIANG Xinyang;LIANG Jinyue;SHI Jitong;LIU Lanling;ZHANG Shanshan;ZHAO Pan;YAN Peizheng;ZHAO Dongsheng(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Experimental Center,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)
出处
《食品工业科技》
CAS
北大核心
2022年第13期381-388,共8页
Science and Technology of Food Industry
基金
国家自然科学基金(81903780,82004233)
国家重点研发计划项目(2017YFC1701503)
山东省中医药科技发展计划项目(2019-0030)
山东省大学生创新创业训练计划项目(S202110441014)
山东中医药大学中药资源保护与质量评价青创团队支持项目。
关键词
补骨脂
乙醇提取物
抗氧化
胆酸盐
关联分析
降血脂
Psoralea corylifolia L.
ethanol extracts
antioxidant
cholate
correlation analysis
hypolipidemic
