摘要
目的建立基于尿酸酶-钯纳米粒子(MCA-Pd NPs)的比色分析法进行临床血清尿酸的定量检测。方法应用比色分析方法检测吸光度值,通过专一性试验、线性范围确定、重复性试验和回收试验等对建立的检测体系进行方法学评价,并检测40份不同尿酸水平的临床血清标本,与生化分析仪结果进行比较。结果检测体系对尿酸具有较高的特异度和选择性;在50.0~1000.0μmol/L浓度范围内线性关系良好;批内和批间精密度分别为2.86%~3.51%和5.12%~7.63%;回收率为97.0%~104.0%;40份临床血清标本检测结果与生化分析仪检测结果线性相关回归方程为Y_(生化分析仪)=0.9964X_(新建方法)-0.4754,r=0.9958。结论所建立的方法对尿酸选择性高,线性范围宽,重复性好,准确度高,能够满足临床分析要求,与临床常规测定血清尿酸的结果具有较好的可比性。
Objective To establish a colorimetric analysis method basing on uricase-palladium nanoparticles(MCA-Pd NPs)for quantitative detection of clinical serum uric acid.Methods Absorbance values were detected by colorimetric analysis method.The performance verification of the establishing method was determined by the specificity test,linear range,repeatability and recovery experiment.A total of 40 clinical serum specimens with different level of uric acid were detected and compared with the results of biochemical analyzer.Results The detection system had high specificity and selectivity for uric acid.A good linear relationship was found among the concentration range of 50.0-1000.0μmol/L.Within-run and between-run precisions were 2.86%-3.51%and 5.12%-7.63%,respectively.The recovery rate was 97.0%-104.0%.Forty clinical serum samples were tested by the establishing method and uricase colorimetric method,and the linear regression equation was Y_(BA)=0.9964X_(new method)-0.4754,r=0.9958.Conclusion The established method has a high specificity,wide linear range,good repeatability,high accuracy and can meet the requirements of clinical analysis,and has good comparability with clinical routine detection method of serum uric acid.
作者
邹瑟音
雷达
凌连生
黄文秀
闫俊平
ZOU Seyin;LEI Da;LING Liansheng;HUANG Wenxiu;YAN Junping(Department of Laboratory Medicine,Guangdong Second Provincial General Hospital,Guangzhou,Guangdong 510000,China;School of Chemical Engineering,Sun Yat-sen University,Guangzhou,Guangdong 510000,China)
出处
《国际检验医学杂志》
CAS
2021年第6期653-655,660,共4页
International Journal of Laboratory Medicine
基金
广东省中医药局课题面上项目(20202004)
广东省第二人民医院青年科研项目(YQ2017-008)。
关键词
钯纳米粒子
尿酸
方法建立
palladium nanoparticles
uric acid
method establishment
