摘要
针对用于血清中尿酸含量检测的荧光纳米材料存在成本高和毒性大的问题,提出一种新型荧光纳米材料的制备。探讨了该荧光纳米材料用于血清中尿酸检测的可行性,对尿酸检测条件进行优化,并对荧光纳米材料检测尿酸的准确度和实际应用效果进行研究。结果表明:在反应温度为15℃、pH值为7.0的醋酸-醋酸钠缓冲液中反应2 min,尿酸对荧光纳米材料荧光强度猝灭率超过90%,其余金属阳离子和小分子对荧光纳米材料的猝灭率均小于5%。在浓度0.1~50μmol/L时,尿酸浓度与荧光纳米材料荧光强度猝灭率相关系数(R^(2))为0.99,检测限(LOD)为28nmol/L;与其他同类检测方法进行对比,荧光纳米材料检测尿酸的检测限较低,表现出较好的选择性和检测性能。用于实际血清样品分析,加标浓度为5、10和30μmol/L时,样品平均加标回收率为98.2%~101.1%,相对标准偏差(RSD)均未超过3%,表现出良好加标回收效果。
A new type of fluorescent nanomaterial was prepared to address the issues of high cost and high toxicity of fluorescent nanomaterials commonly used for detecting uric acid content in serum.The feasibility of using this fluorescent nanomaterial for the detection of uric acid in serum was explored,then the detection conditions for uric acid were optimized,and finally the accuracy and practical application effect of fluorescent nanomaterials for detecting uric acid were studied.The experimental results showed that after 2 minutes of reaction in acetic acid sodium acetate buffer at a reaction temperature of 15℃and pH value of 7.0,the quenching rate of uric acid on the fluorescence intensity of fluorescent nanomaterials exceeded 90%,while the quenching rate of other metal cations and small molecules on fluorescent nanomaterials was less than 5%.Within the linear range of 0.1 and 50μmol/L,the correlation coefficient R^(2) between uric acid concentration and fluorescence intensity quenching rate of fluorescent nanomaterials was 0.99,and the detection limit(LOD)was 28 nmol/L.Compared with other similar detection methods,fluorescent nanomaterials had a lower detection limit for detecting uric acid,exhibiting better selectivity and detection performance.When used for actual serum sample analysis with a spiked concentration of 5μmol/L,10μmol/L and 30μmol/L,the average spiked recovery rate of the sample ranged from 98.2%to 101.1%,and the relative standard deviation(RSD)did not exceed 3%,demonstrating a good spiked recovery effect.
作者
钟国华
廖继梅
彭方毅
姜海蓉
钟诗淇
ZHANG Guohua;LIAO Jimei;PENG Fangyi;JIANG Hairong;ZHONG Shiqi(Xinglong Town Central Health Center,Fengjie County,Chongqing,Chongqing 404604,China;Chongqing Leibo Medical Laboratory,Chongqing 400030,China;Chongqing Public Health Treatment Center,Chongqing 400030,China;Southwest University,Chongqing 400715,China)
出处
《粘接》
CAS
2023年第9期98-101,127,共5页
Adhesion
关键词
尿酸检测
荧光纳米材料
化学合成
荧光强度
临床检验
uric acid testing
fluorescent nanomaterials
fluorescence intensity
clinical testing
chemical synthesis
