摘要
病毒病是引起兰州百合产量下降、品种退化的主要原因,准确、高效的病毒检测技术在百合脱毒种球生产和推广应用中十分重要。根据CMV、LSV和LMoV病毒外壳蛋白基因序列设计引物,以百合18S rRNA为内参照,建立兰州百合病毒RT-PCR、二重RT-PCR检测体系。对兰州百合主栽区病毒病的抽样调查发现,CMV、LSV带毒率分别达到98%、100%,LMoV检出率在不同时期样品中存在较大差异。
Virus disease is the main cause of yield decline and variety degradation of Lilium davidii Var.unicolor.It is very important to master efficient virus detection technologies in the production of virus-free bulb seedball and planting period.The PCR primers were designed according to the sequences of CMV,LSV and LMoV virus coat protein genes,and the virus detection system of RT-PCR and double RT-PCR was established with lily 18S rRNA as internal reference in this study.The results showed that sampling survey of Lilium davidii Var.unicolor.virus disease in main planting area,CMV and LSV were 98%and 100%respectively,and detection rates of LMoV were different in different periods samples.
作者
王红梅
王立光
刘新星
李淑洁
石有太
李忠旺
WANG Hongmei;WANG Liguang;LIU Xinxing;LI Shujie;SHI Youtai;LI Zhongwang(Institute of Biotechnology,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China)
出处
《甘肃农业科技》
2021年第3期38-43,共6页
Gansu Agricultural Science and Technology
基金
甘肃省农业科学院科技支撑计划(No.2018GAAS01)
甘肃省农业科学院重点研发计划(No.2019GAAS019)
兰州市科技计划项目(No.2019-1-67)。
